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Human vascular injury 1 kit

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The Human Vascular Injury I Kit is a laboratory equipment product designed for the study of vascular injury in human tissue samples. It provides the necessary tools and components to conduct controlled experiments and analysis related to vascular damage and repair mechanisms.

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Lab products found in correlation

V plex human proinflammatory panel, by Mesoscale (2 mentions) Human mmp 3 plex ultra sensitive kit, by Mesoscale (1 mentions)

Close spelling variants of this product

Vascular Injury Panel 1 Human Kit

3 protocols using human vascular injury 1 kit

1

Biomarker Dynamics in ECMO Patients

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Blood samples were obtained daily during ECMO from the ECMO circuit or existing indwelling catheters, at the same time as routine clinical blood draws. Plasma was separated by centrifugation and stored at −80°C. Plasma concentrations for interferon gamma (IFNγ), interleukin-6 (IL-6), interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNFα), E-selectin, P-selectin, intercellular adhesion molecule-3 (ICAM-3), thrombomodulin (TM), tissue plasminogen activator (tPA), and plasminogen activator inhibitor-1 (PAI-1), were measured for ECMO days 1, 2, 3 and 5. Measurements for ECMO day 7 and every third day thereafter until ECMO decannulation were conducted if ECMO duration was longer than 5 days.
All assays were conducted in the Johns Hopkins Institute for Clinical and Translational Research (ICTR) Clinical Research Unit: Core Laboratory, using the Meso Scale Discovery (Rockville, MD) V-PLEX Human Proinflammatory Panel (for IFNγ, IL-6, IL-1β, TNFα), the Human Vascular Injury I Kit (for E-selectin, P-selectin, ICAM-3, TM), and single plex assays for tPA and PAI-1. Dynamic ranges for each analyte have been published.21 -23 The inter-assay coefficients of variation for the two multiplex assays used were: IFNγ, 7.96; IL-6, 15.93; IL-1β, 0.02; TNFα, 5.21; E-selectin, 6.45; P-selectin, 14.39; ICAM-3, 11.78; TM, 8.6. Laboratory personnel were blinded to clinical data.
Caprarola S.D., Ng D.K., Carroll M.K., Tekes A., Felling R.J., Salorio C.F., Almuqati R., Schwartz J.M., Everett A.D, & Bembea M.M. (2021). Pediatric ECMO: Unfavorable Outcomes are Associated with Inflammation and Endothelial Activation. Pediatric research, 92(2), 549-556.
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2

Multiplex Cytokine and Matrix Metalloproteinase Profiling

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Twenty-one markers were measured using the following Mesoscale Discovery kits: Human Vascular Injury I Kit, V-PLEX Vascular Injury Panel 2 Human Kit, V-PLEX Human Proinflammatory Panel II + IFN-γ, Human MMP 3-Plex Ultra-Sensitive Kit, and a custom U-PLEX panel for M-CSF, IL-4, MCP-1, Fractalkine, and VEGF-A. Manufacturer instructions were followed for each kit. All samples were bound to plates overnight at 4 °C with vigorous agitation on a MicroMix 5 plate shaker (DPC). Lysates were diluted in the recommended diluent provided with each kit. Of the 21 markers, IL-4 had 65% of the samples below detection limit and ICAM-3 signal was very low and close to background, so neither analyte was included in further analysis.
Saul J., Hutchins E., Reiman R., Saul M., Ostrow L.W., Harris B.T., Van Keuren-Jensen K., Bowser R, & Bakkar N. (2020). Global alterations to the choroid plexus blood-CSF barrier in amyotrophic lateral sclerosis. Acta Neuropathologica Communications, 8, 92.
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3

Biomarker Dynamics in ECMO Patients

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Blood samples were obtained daily during ECMO from the ECMO circuit or existing indwelling catheters, at the same time as routine clinical blood draws. Plasma was separated by centrifugation and stored at −80°C. Plasma concentrations for interferon gamma (IFNγ), interleukin-6 (IL-6), interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNFα), E-selectin, P-selectin, intercellular adhesion molecule-3 (ICAM-3), thrombomodulin (TM), tissue plasminogen activator (tPA), and plasminogen activator inhibitor-1 (PAI-1), were measured for ECMO days 1, 2, 3 and 5. Measurements for ECMO day 7 and every third day thereafter until ECMO decannulation were conducted if ECMO duration was longer than 5 days.
All assays were conducted in the Johns Hopkins Institute for Clinical and Translational Research (ICTR) Clinical Research Unit: Core Laboratory, using the Meso Scale Discovery (Rockville, MD) V-PLEX Human Proinflammatory Panel (for IFNγ, IL-6, IL-1β, TNFα), the Human Vascular Injury I Kit (for E-selectin, P-selectin, ICAM-3, TM), and single plex assays for tPA and PAI-1. Dynamic ranges for each analyte have been published.21 -23 The inter-assay coefficients of variation for the two multiplex assays used were: IFNγ, 7.96; IL-6, 15.93; IL-1β, 0.02; TNFα, 5.21; E-selectin, 6.45; P-selectin, 14.39; ICAM-3, 11.78; TM, 8.6. Laboratory personnel were blinded to clinical data.
Caprarola S.D., Ng D.K., Carroll M.K., Tekes A., Felling R.J., Salorio C.F., Almuqati R., Schwartz J.M., Everett A.D, & Bembea M.M. (2021). Pediatric ECMO: Unfavorable Outcomes are Associated with Inflammation and Endothelial Activation. Pediatric research, 92(2), 549-556.
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