All the studies using nasal septal chondrocytes (NSCs) were conducted after written approval (HC13TISI0038) obtained from the Institutional Review Board of the Catholic Medical Center Clinical Research Coordinating Center. Human nasal septum tissue was harvested from five patients who were undergoing septoplasty [21 (link)]. Those tissues were cut into 1 mm3 pieces and they were enzymatically digested using 0.2 % protease solution (Gibco) for 60 min, followed by incubation in 0.3 % collagenase (Sigma-Aldrich) for 12 h at 37 °C. The isolated cells were then seeded in a 75-mm2 cell culture flask (NUNC) and cultivated in low-glucose Dulbecco’s Modified Eagle Medium (Gibco-BRL) with 10 % fetal bovine serum (FBS; Gibco) and antibiotics at 37 °C in 5 % CO2 incubator. The confluent chondrocytes were subcultured following a standard protocol using 0.05 % trypsin/EDTA solution (Gibco).
Protease solution
Manufactured by Thermo Fisher Scientific
Protease solution is a laboratory reagent that contains enzymes capable of breaking down proteins. It is commonly used in various biological and biochemical applications to facilitate the digestion or dissociation of protein samples.
Automatically generated - may contain errors
Lab products found in correlation
Collagenase, by Merck Group (2 mentions)
75 mm2 cell culture flask, by Thermo Fisher Scientific (2 mentions)
Low glucose dulbecco s modified eagle medium, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Trypsin edta solution, by Thermo Fisher Scientific (2 mentions)
2 protocols using protease solution
1
Isolation and Cultivation of Nasal Septal Chondrocytes
2
Isolation and Culture of Human Nasal Septal Chondrocytes
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All the studies using nasal septal chondrocytes (NSCs) were conducted after written approval (HC13TISI0038) obtained from the Institutional Review Board of the Catholic Medical Center Clinical Research Coordinating Center. Human nasal septum tissue was harvested from five patients who were undergoing septoplasty [21] .
Those tissues were cut into 1 mm 3 pieces and they were enzymatically digested using 0.2% protease solution (Gibco) for 60 min, followed by incubation in 0.3% collagenase (Sigma-Aldrich) for 12hr at 37 °C. The isolated cells were then seeded in a 75-mm 2 cell culture flask (NUNC) and cultivated in low-glucose Dulbecco's Modified Eagle Medium (Gibco-BRL) with 10% fetal bovine serum (FBS; Gibco) and antibiotics at 37 °C in 5% CO 2 incubator. The confluent chondrocytes were subcultured following a standard protocol using 0.05% trypsin/EDTA solution (Gibco).
Those tissues were cut into 1 mm 3 pieces and they were enzymatically digested using 0.2% protease solution (Gibco) for 60 min, followed by incubation in 0.3% collagenase (Sigma-Aldrich) for 12hr at 37 °C. The isolated cells were then seeded in a 75-mm 2 cell culture flask (NUNC) and cultivated in low-glucose Dulbecco's Modified Eagle Medium (Gibco-BRL) with 10% fetal bovine serum (FBS; Gibco) and antibiotics at 37 °C in 5% CO 2 incubator. The confluent chondrocytes were subcultured following a standard protocol using 0.05% trypsin/EDTA solution (Gibco).
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