Animals were euthanized and fixed by intracardiac perfusion with a 4% paraformaldehyde solution. Tongues were excised and placed in 30% sucrose solution overnight at 4°C for cryoprotection. Tissues were embedded in OCT compound and sectioned at 30 μm thickness on a cryostat. Sections were washed in PBS with 0.1% Triton X-100 (PBST), blocked with 10% donkey serum in PBST, incubated with primary antibodies overnight at 4°C, and incubated with fluorescence-tagged secondary antibodies (Jackson ImmunoResearch) for 2 hours at room temperature. Primary antibodies used were: anti-Sema7A (R&D Systems #AF1835; 1:300 dilution), anti-FLAG (Sigma #F7425; 1:1000 dilution), anti-T1R3 (Santa Cruz Biotechnology #sc-22458; 1:500 dilution), anti-Car4 (R&D Systems #AF2414; 1:500 dilution), anti-Plcβ2 (Santa Cruz Biotechnology #sc-206; 1:1000 dilution), anti-GFP (Abcam #ab13970; 1:500 dilution), anti-Nrp1 (R&D Systems #AF566; 1:200 dilution), and anti-PlxnC1 (R&D Systems #AF5375; 1:200 dilution).
Sc 22458
Manufactured by R&D Systems
Sc-22458 is a laboratory equipment product from R&D Systems. It is a specialized tool designed for scientific research and experimentation purposes. The core function of Sc-22458 is to facilitate specific tasks or measurements required in a laboratory setting. Detailed specifications and intended use of this product are not available.
Automatically generated - may contain errors
Lab products found in correlation
Ab13970, by R&D Systems (2 mentions)
Af566, by R&D Systems (2 mentions)
Fluorescence tagged secondary antibodies, by Jackson ImmunoResearch (2 mentions)
Af1835, by Merck Group (2 mentions)
Af2414, by Santa Cruz Biotechnology (2 mentions)
Sc 206, by Abcam (2 mentions)
Af5375, by R&D Systems (2 mentions)
F7425, by Merck Group (2 mentions)
2 protocols using sc 22458
1
Immunofluorescence Characterization of Taste Receptors
2
Immunofluorescence Characterization of Taste Receptors
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Animals were euthanized and fixed by intracardiac perfusion with a 4% paraformaldehyde solution. Tongues were excised and placed in 30% sucrose solution overnight at 4°C for cryoprotection. Tissues were embedded in OCT compound and sectioned at 30 μm thickness on a cryostat. Sections were washed in PBS with 0.1% Triton X-100 (PBST), blocked with 10% donkey serum in PBST, incubated with primary antibodies overnight at 4°C, and incubated with fluorescence-tagged secondary antibodies (Jackson ImmunoResearch) for 2 hours at room temperature. Primary antibodies used were: anti-Sema7A (R&D Systems #AF1835; 1:300 dilution), anti-FLAG (Sigma #F7425; 1:1000 dilution), anti-T1R3 (Santa Cruz Biotechnology #sc-22458; 1:500 dilution), anti-Car4 (R&D Systems #AF2414; 1:500 dilution), anti-Plcβ2 (Santa Cruz Biotechnology #sc-206; 1:1000 dilution), anti-GFP (Abcam #ab13970; 1:500 dilution), anti-Nrp1 (R&D Systems #AF566; 1:200 dilution), and anti-PlxnC1 (R&D Systems #AF5375; 1:200 dilution).
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