Iron acetylacetonate feaa catalyst

DMEM (1.0 g/L glucose = 1.0 g/L) and fetal bovine serum (FBS) were purchased from Thermo Scientific. Penicillin/streptomycin (P/S), trypsin EDTA, and Amphotericin B were obtained from Corning Cellgro. Glycolide and D,L-lactide were purchased from Polysciences (Warrington, PA). Hexamethylene diisocyanate trimer (HDIt) was supplied by Bayer Material Science (Pittsburgh, PA). Iron acetylacetonate (FeAA) catalyst and Dorsomorphin was supplied by Sigma-Aldrich. ε-caprolactone (Sigma-Aldrich) was dried over anhydrous MgSO₄, and all other materials were used as received.

nHA particles were purchased from Sigma-Aldrich and dried at 80 °C under vacuum for 48 hours before use. LTI was supplied by Jinan Haohua Industry Co., Ltd (Jinan, China). Before use, LTI was purified by dissolving in tert-butyl methyl ether (TBME, Across-Organic) and refluxing with decolorizing carbon (Fisher Scientific) at 63°C for 22 hours. α-MEM, FBS, trypsin-EDTA and Antibiotic-Antimycotic were purchased from ThermoFisher Scientific. Mesenchymal stem cell media were purchased from PromoCell, Inc. Iron acetylacetonate (FeAA) catalyst, fibronectin, vitronectin, Alizarin Red S and 1α,25-Dihydroxyvitamin D3 were purchased from Sigma-Aldrich and used as received. The mouse MC3T3 osteoblast cell line was purchased from ATCC. Human bone marrow derived mesenchymal stem cells (hMSCs) were purchased from Extem Bioscience (San Francisco, CA). Dentin was acquired from the donation of a surrendered elephant tusk from the National Eagle and Wildlife Property Repository of the United States Department of the Interior, U.S. Fishes and Wildlife Service.