The cells were homogenized in lysis buffer (50 mM/L HEPES pH 7.5, 150 mM/L NaCl, 10% glycerol, 1% Triton X-100, 1 mM/L EGTA, 1.5 mM/L MgCl2, 10 mM/L NaF, 10 mM/L sodium pyrophosphate, 1mM/L Na3VO4, 10 μg approtinin/ml, 10 μg leupeptin/ml) (Sigma). The following antibodies were used: α-RAS, α-RAC, α-CDC42, α-RHO, α-pEGFR, α-EGFR, α-pFAK, α-FAK, α-pSCR, α-SCR, α-pMEK, α-MEK, α-pERK1/2, α-ERK1/2, α-pAKT, α-AKT, α-pGSK3 α /β, α-GSK3 α /β, α-β catenin, α-pCHK2, α-CHK2, α-tubulin, α-SP1, α-pRET, α-RET (Cell Signaling, Danvers, MA, USA), and α-SOD3 (Santa Cruz, Santa Cruz, CA, USA). Signal density analysis was performed using ImageJ software GEL blot software.
α fak
Manufactured by Cell Signaling Technology
Sourced in United States
α-FAK is a primary antibody that recognizes the focal adhesion kinase (FAK) protein. FAK is a non-receptor tyrosine kinase that plays a crucial role in the regulation of cell adhesion, migration, and survival. The α-FAK antibody can be used to detect and analyze the expression and activation of FAK in various cellular and experimental contexts.
Automatically generated - may contain errors
Lab products found in correlation
α akt, by Cell Signaling Technology (2 mentions)
α erk1 2, by Cell Signaling Technology (2 mentions)
α pchk2, by Cell Signaling Technology (1 mentions)
α egfr, by Cell Signaling Technology (1 mentions)
α mek, by Cell Signaling Technology (1 mentions)
α perk1 2, by Cell Signaling Technology (1 mentions)
α β catenin, by Cell Signaling Technology (1 mentions)
α sp1, by Cell Signaling Technology (1 mentions)
α tubulin, by Cell Signaling Technology (1 mentions)
Leupeptin, by Merck Group (1 mentions)
Defactinib, by Selleck Chemicals (1 mentions)
Ab181548, by Abcam (1 mentions)
Dasatinib, by Selleck Chemicals (1 mentions)
α gapdh, by Cell Signaling Technology (1 mentions)
α tubulin dm1a, by Merck Group (1 mentions)
Ab181434, by Abcam (1 mentions)
Ab2413, by Abcam (1 mentions)
α src, by Cell Signaling Technology (1 mentions)
α phospho erk1 2, by Cell Signaling Technology (1 mentions)
α phospho akt, by Cell Signaling Technology (1 mentions)
2 protocols using α fak
1
Western Blot Analysis of Cell Signaling
2
Integrin-Mediated Signaling Pathway Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Primary antibodies used in this study include: α-fibronectin (ab2413; Abcam), α-tubulin (DM1A; Sigma-Aldrich), α-GAPDH (1:2500, 14C10; Cell Signaling Technology), α-collagen IV (1:100, ab6586; Abcam), α-phospho-Src (2101; Cell Signaling Technology), α-Src (36D10; Cell Signaling Technology), α-phospho-FAK (3283; Cell Signaling Technology), α-FAK (D5O7U; Cell Signaling Technology), α-phospho-Erk1/2 (4370; Cell Signaling Technology), α-Erk1/2 (4695; Cell Signaling Technology), α-phospho-Akt (9271; Cell Signaling Technology), α-Akt (4691; Cell Signaling Technology), α-integrin α1 (ITGA1; ab181434; Abcam), and α-integrin α2 (ITGA2; ab181548; Abcam) All antibodies were used at a concentration of 1:1000 unless indicated otherwise. Pharmacologic inhibitors include: dasatinib (S1021; Selleck Chemicals) and defactinib (S7654; Selleck Chemicals).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!