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Elisa ready set go elisa kits

Manufactured by Thermo Fisher Scientific
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The ELISA Ready-SET-Go!® ELISA kits are pre-coated enzyme-linked immunosorbent assay (ELISA) components designed for the quantitative measurement of specific proteins or other analytes in a variety of sample types.

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2 protocols using elisa ready set go elisa kits

1

Quantifying Mouse Cytokine Responses

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Mouse IL-10, IL-1β, IL-6 and TNF-α ELISA Ready-SET-Go!® ELISA kits (eBiosciences) were used according to the manufacturers’ instructions. Quantitative PCR (qPCR) was performed using primers for IL-10 (forward, 5′-CCAAGCCTTATCGGAAATGA-3′ and reverse, 5′-TCCTGAGGGTCTTCAGCTTC-3′), and β-2m (forward, 5′-TCAGTCGCGGTCGCTTC-3′ and reverse, 5′-CAAGCACCAGAAAGACTAGG GTC-3′). Fold induction was calculated using MB1cre/+ cells cultured in media as a reference.
Pore D., Matsui K., Parameswaran N, & Gupta N. (2015). Ezrin regulates inflammation by limiting B cell interleukin-10 production. Journal of immunology (Baltimore, Md. : 1950), 196(2), 558-562.
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2

Quantification of Tumor Immune Markers

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Tumor lysates were collected from tumor-bearing mice that underwent the indicated treatments at the indicated time points. The levels of IL-12 and IFNγ were measured by using ELISA Ready-SET-Go ELISA kits (eBioscience, San Diego, CA). The levels of human CXCL9 and CXCL10 in xenograft tumors were measured by using ELISA kits (LSBio, Seattle, WA).
Hu J., Sun C., Bernatchez C., Xia X., Hwu P., Dotti G, & Li S. (2018). T cell homing therapy for reducing regulatory T cells and preserving effector T cell function in large solid tumors. Clinical cancer research : an official journal of the American Association for Cancer Research, 24(12), 2920-2934.
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