Acrylate peg n hydroxysuccinimide

The fibronectin-mimetic cell adhesive peptide sequence Arg-Gly-Asp-Ser (RGDS; 433 Da, EMD Chemicals, San Diego CA) was coupled to acrylate-PEG-N-Hydroxysuccinimide (MW 3500 Da, Jenkem Technology, Beijing China) through the amino terminus, as previously described, allowing it to be tethered into hydrogels via copolymerization with PEGPLA_mDM (43 ). The product (Ac-PEG-RGDS, Fig. 1) was dialyzed against deionized water (molecular weight cutoff = 1000 Da, Spectrum Labs, Rancho Dominguez CA), lyophilized, analyzed via matrix-assisted laser desorption/ionization-time of flight mass spectrometry (Fig. S1, MALDI-TOF, Bruker AutoFlex III SmartBeam) (solvent: 50% acetonitrile in H₂O + 0.1% TFA; matrix: α-cyano-4-hydroxy cinnamic acid (TCI Europe); calibrant: Peptide Calibration Standard (Bruker)) (m/z + Cl⁻, 3854 Da), and stored at 4 °C.

The cell adhesive sequence Arg-Gly-Asp-Ser (Fig. 1; RGDS; 433 Da, EMD Chemicals, San Diego CA) was coupled to acrylate-PEG-N-Hydroxysuccinimide (Jenkem Technology, Beijing China, MW 3500 Da, p=79) through the amino terminus to allow for incorporation into hydrogels, as previously described [12 (link), 51 ]. The product (Acrylate-PEG-RGDS, Fig. 1) was dialyzed against deionized water (molecular weight cutoff = 1000 Da, Spectrum Labs, Rancho Dominguez CA), lyophilized, analyzed via matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF, Bruker AutoFlex III SmartBeam); (solvent: 50% acetonitrile in H₂O + 0.1% TFA; matrix: α-cyano-4-hydroxy cinnamic acid (TCI Europe); calibrant: Peptide Calibration Standard (Bruker, #206195)) (m/z Cl-, 3854 Da), and stored at 4 °C.