Human USC were cultured in combined media: Keratinocyte serum-free medium (KSFM) and progenitor cell medium (1:1) as previously reported [55 (link)]. Briefly, KSFM was supplemented with 5 ng/ml epidermal growth factor, 50 ng/ml bovine pituitary extract, 30 ng/ml cholera toxin, 100 U/ml penicillin and 1 mg/ml streptomycin. Progenitor cell medium contained ¾ Dulbecco’s modified Eagle’s medium, ¼ Hamm’s F12, 10% fetal bovine serum (FBS), 0.4 μg/ml hydrocortisone, 10−10 M cholera toxin, 5 ng/ml insulin, 1.8 × 10−4 M adenine, 5 μg/ml transferrin plus 2 × 10−9 M 3,39,5-triiodo-L -thyronine, 10 ng/ml epidermal growth factor (EGF), 10% penicillin and streptomycin, were all purchased from Gibco (Thermo Fisher Scientific, Waltham, MA, USA). Acetone, ethanol, methanol, isopropanol, phosphate buffered saline (PBS) and all other reagents were used in this study. Demineralized water was used in all cases.
Hamm s f12
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Hamm's F12 is a laboratory equipment designed for cell culture applications. It functions as a specialized incubator to provide a controlled environment for the growth and maintenance of cells.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (6 mentions)
Penicillin, by Thermo Fisher Scientific (4 mentions)
Streptomycin, by Thermo Fisher Scientific (4 mentions)
Hydrocortisone, by Thermo Fisher Scientific (2 mentions)
Insulin, by Thermo Fisher Scientific (2 mentions)
Transferrin, by Thermo Fisher Scientific (2 mentions)
Adenine, by Thermo Fisher Scientific (2 mentions)
Cholera toxin, by Thermo Fisher Scientific (2 mentions)
3 39 5 triiodo l thyronine, by Thermo Fisher Scientific (2 mentions)
Epidermal growth factor (egf), by Thermo Fisher Scientific (2 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (2 mentions)
2 protocols using hamm s f12
1
Culturing Human USC in Combined Media
2
Culturing Human USC in Combined Media
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human USC were cultured in combined media: Keratinocyte serum-free medium (KSFM) and progenitor cell medium (1:1) as previously reported [55 (link)]. Briefly, KSFM was supplemented with 5 ng/ml epidermal growth factor, 50 ng/ml bovine pituitary extract, 30 ng/ml cholera toxin, 100 U/ml penicillin and 1 mg/ml streptomycin. Progenitor cell medium contained ¾ Dulbecco’s modified Eagle’s medium, ¼ Hamm’s F12, 10% fetal bovine serum (FBS), 0.4 μg/ml hydrocortisone, 10−10 M cholera toxin, 5 ng/ml insulin, 1.8 × 10−4 M adenine, 5 μg/ml transferrin plus 2 × 10−9 M 3,39,5-triiodo-L -thyronine, 10 ng/ml epidermal growth factor (EGF), 10% penicillin and streptomycin, were all purchased from Gibco (Thermo Fisher Scientific, Waltham, MA, USA). Acetone, ethanol, methanol, isopropanol, phosphate buffered saline (PBS) and all other reagents were used in this study. Demineralized water was used in all cases.
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