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Mouse anti py 4g10

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Mouse anti-pY 4G10 is a laboratory reagent used for the detection of phosphorylated tyrosine residues in proteins. It is a mouse monoclonal antibody that specifically binds to phosphorylated tyrosine epitopes, allowing for the identification and analysis of tyrosine phosphorylation events in biological samples.

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Lab products found in correlation

Indo 1 am, by Thermo Fisher Scientific (1 mentions) Mouse anti ha ha 7, by Merck Group (1 mentions) Mouse anti flag m2, by Merck Group (1 mentions) Mouse anti actin, by Merck Group (1 mentions) Rabbit anti lck, by Cell Signaling Technology (1 mentions) Mouse anti cd3ζ 6b 10, by Santa Cruz Biotechnology (1 mentions)

2 protocols using mouse anti py 4g10

1

T Cell Activation Signaling Pathway

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Human anti-CD3ε antibodies (BD Pharmingen) were used to coat coverslips. The following antibodies were used: rabbit anti-CD4 (Sigma), mouse anti-LAT (Upstate), rabbit anti-pLAT191 (Invitrogen), mouse anti- PLCγ1, mouse SLP-76 (Antibody Solutions), mouse anti-pY 4G10 (Millipore). Alexa-488 or APC-conjugated anti-CD4 [clones OKT4 and SK3 (eBioscience)] were used for labeling CD4. Indo-1 AM was from Invitrogen. TAK-243 to inhibit E1 ubiquitin activating enzyme was from Selleckchem.
Balagopalan L., Malik H., McIntire K.M., Garvey J.A., Nguyen T., Rodriguez-Peña A.B, & Samelson L.E. (2020). Bypassing ubiquitination enables LAT recycling to the cell surface and enhanced signaling in T cells. PLoS ONE, 15(2), e0229036.
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2

Western Blotting Analysis of Key Signaling Proteins

Cited 1 time
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Abs used for Western blotting analysis were: mouse anti-Csk (BD Biosciences), rabbit anti-Csk (C-20), rabbit anti-TRAF3 (H122,), mouse anti-CD3ζ (6B10.2, all from Santa Cruz), rabbit anti-pCsk S364 (Abcam), rabbit anti-PTPN22 (D6D1H), rabbit anti-pSrc 416, rabbit anti-Lck, rabbit anti-pLck 505, rabbit anti-LAT (all from Cell Signaling), mouse anti-FLAG (M2), mouse anti-HA (HA-7, all from Sigma), mouse anti-pY (4G10) and mouse anti-actin (Clone 4, Millipore). Western blot analysis was performed as in ref. 13 (link).
Wallis A.M., Wallace E.C., Hostager B.S., Yi Z., Houtman J.C, & Bishop G.A. (2017). TRAF3 enhances TCR signaling by regulating the inhibitors Csk and PTPN22. Scientific Reports, 7, 2081.
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