Freshly cut TMA sections were all stained for CD8 in one run on the same day. The slides were deparaffinized, rehydrated, exposed to heat-induced antigen retrieval for 15 min at 98 °C in pH 9 DAKO target retrieval Solution (S2367) using a DAKO PT-LINK device, and then transferred to a DAKO Link 48 autostainer device. The autostainer protocol includes peroxidase blocking for 5 min (DAKO, Envision Flex-Kit 8002) and subsequent incubations with the primary antibody (Oncodianova, mouse monoclonal antibody, Clone TC8, dilution 1:200) for 20 min at room temperature, Flex HRP (DAKO Envision Flex-Kit 8002) for 20 min, DAB-Chromogen (DAKO Envision Flex-Kit 8002) for 10 min, and a final incubation with Hämatoxylin (DAKO K8008) for 5 min.
Blessin N.C., Spriestersbach P., Li W., Mandelkow T., Dum D., Simon R., Hube-Magg C., Lutz F., Viehweger F., Lennartz M., Fraune C., Nickelsen V., Fehrle W., Göbel C., Weidemann S., Clauditz T., Lebok P., Möller K., Steurer S., Izbicki J.R., Sauter G., Minner S., Jacobsen F., Luebke A.M., Büscheck F., Höflmayer D., Wilczak W., Burandt E, & Hinsch A. (2020). Prevalence of CD8+ cytotoxic lymphocytes in human neoplasms. Cellular Oncology (Dordrecht), 43(3), 421-430.
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