After the root exudates were collected, the glass beads were gently removed from the tubes, to prevent damaging the roots. Next, the roots were cut at the mesocotyl to separate roots from shoots. Roots and shoots were weighed separately, and roots scanned individually. Each root was placed on the scanner screen (Epson Perfection V800 Photo scanner, Epson America, Inc.) and moistened with 1 mM CaCl2 and manually spread to separate the different types of roots. To retrieve the images, the SilverFast SE software was used (LaserSoft Imaging, Inc.), and flipped and inverted using Adobe Photoshop. The pictures were submitted to the website of DIRT software for root trait analyses [39 (link)]. The masking threshold was set to 10.0 to remove the background noise and all the default settings. The shoots were photographed for image analysis and for a record of plant health. Only the relevant data retrieved by DIRT was shown in the results.
Perfection v800 photo scanner
Manufactured by Epson
Sourced in Japan
The Epson Perfection V800 Photo scanner is a flatbed scanner designed for professional-quality photo and film scanning. It features a resolution of up to 6400 dpi and can handle a variety of media including 35mm, medium format, and 4x5 inch film.
Automatically generated - may contain errors
Lab products found in correlation
Winrhizo, by Regent Instruments (2 mentions)
Crystal violet, by Merck Group (2 mentions)
Winfolia software, by Regent Instruments (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
Axio zoom v16 microscope, by Zeiss (1 mentions)
Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions)
Formaldehyde, by Thermo Fisher Scientific (1 mentions)
Winrhizo image analysis system, by Regent Instruments (1 mentions)
18 protocols using perfection v800 photo scanner
1
Root Scanning and Trait Analysis
2
Arabidopsis Root Response to Heterodera Inoculation
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Individual Arabidopsis seeds were sown in square Petri dishes. Nine‐day‐old seedlings were inoculated with 0 (mock), 50, 100, 200, 350, or 500 H. schachtii J2s. Specifically, two 5 μl drops of solution (with J2s or mock) were pipetted at opposite sides of each seedling while keeping the Petri dishes vertical. This allowed for a homogeneous smear of J2s along the whole length of the root. At 7 d post‐inoculation (dpi), scans were made of whole seedlings using an Epson Perfection V800 photo scanner (Epson, Nagano, Japan). The root architecture (total root length, primary root length, and total secondary root length) was measured using the Win Rhizo package for Arabidopsis (Regent Instruments Inc., Québec, Canada). For the coi1‐2 mutant, primary root length was measured manually because of the convoluted root system. The number of root tips was counted manually based on the scans. Furthermore, nematodes within the roots were stained with acid fuchsin and counted as previously described (Warmerdam et al., 2018 (link)). For comparisons between genotypes, the background effect of the mutation on the root architecture was corrected by normalizing each measured component in infected seedlings to the average respective component in mock‐inoculated roots. Additionally, the presence of clusters and the number of secondary roots per cluster were scored using a binocular.
3
Root Surface, Volume, and Length Analysis
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The surface, volume and total length of root were determined by using an
image-analysis technique [55 ]. Roots were washed with distilled water and soaked in water
contained in a transparent tray, then placed on Epson Perfection V800 Photo
scanner (Epson, Long Beach, USA). The digitized images were measured by Winrhizo
(Regent Instruments Inc., Quebec, Canada). The root was oven-dried for 24 h at
80°C, and weighed with electronic analytical balance.
image-analysis technique [55 ]. Roots were washed with distilled water and soaked in water
contained in a transparent tray, then placed on Epson Perfection V800 Photo
scanner (Epson, Long Beach, USA). The digitized images were measured by Winrhizo
(Regent Instruments Inc., Quebec, Canada). The root was oven-dried for 24 h at
80°C, and weighed with electronic analytical balance.
4
DAHP Plaque Assay for Intracellular Parasites
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Intracellular parasites were mechanically-lysed and filtered. 500 parasites of the indicated strain were infected into a well of a 6-well plate in the indicated media. For DAHP plaque assays, wells were then treated with the indicated concentrations of DAHP or PBS vehicle. For BH4 rescue experiments, wells received 50 μM BH4 or the equivalent volume of DMSO vehicle. Plaques were allowed to form for 7 days prior to PBS wash and ethanol fixation. Wells were stained for 10 min with crystal violet (12.5 g crystal violet, 125 ml 100% ethanol, 500 ml 1% ammonium oxalate). Wells were then washed three times with water and allowed to dry overnight. Stained plaques were scanned on an Epson Perfection V800 Photo scanner. All plaque assays were conducted at least twice.
5
Automated Growth Monitoring of Microalgae
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Cells were grown until exponential phase (2-6 x 106 cells mL−1) and serial dilutions in TAP or HSM media were made. The dilution was spotted (10 µL) onto TAP and HSM agar plates at different light intensities. The plates were then scanned after 7 days using a Perfection V800 Photo scanner (Epson). This analysis was automatized using an Opentrons OT-2.
6
Colorimetric qLAMP Assay for Bacterial Detection
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Colorimetric qLAMP assays were performed for 60 min using 1250 copies per reaction of gDNA of one, two, and/or three bacteria P. multocida, M. haemolytica, and H. somni in the same reaction. Each condition was repeated nine times in nine separate wells of 96-well plates. Images of the plates were taken at 0 and 60 min using the Epson Perfection V800 Photo scanner. Colorimetric absorbance ratios were calculated as explained above, and the resulting data were plotted against time. The finalized data were also analyzed in receiver operating characteristic (ROC) curves by using the colorimetric threshold previously determined and assessing positive versus negative reactions for each primer set. The highest number obtained from subtracting the false positive rate (Equation 2 ) from the true positive rate (Equation 3 ) was selected as the time threshold for that specific primer.
7
Time-lapse Imaging of Microbial Growth
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Cells (10 µL) were inoculated onto exploration medium, after which the plates were placed on an Epson Perfection V800 Photo scanner, programmed to acquire one image every hour, and incubated at 30 °C. The time-course images were then compiled to video format as sequential single frames.
8
Cell Survival Assay for MBTPS2 Variants
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Cell survival assays were done as previously described for MBTPS2 (Oeffner et al., 2009 (link)). Briefly, cells stably transfected with pcDNA (control), wild‐type MBTPS2, or variant‐MBTPS2 were plated in a 5.5‐cm dish at a density of 1 × 105 cells in DMEM:F12 supplemented with 10% FBS and 400 μg/ml G418. The following day, media was switched to sterol‐deficient media (DMEM:F12 with 5% lipoprotein‐deficient serum and 400 μg/ml G418) or sterol‐enriched media (DMEM:F12 with 5% lipoprotein‐deficient serum, 5 μg/ml water‐soluble cholesterol, 100 μM sodium mevalonate, 20 μM sodium oleate, and 400 μg/ml of G418). Media was refreshed on Day 3. On Day 6, cells were harvested for quantitation or staining. Cell quantitation was done via counting with a hemocytometer. For staining studies, cells were washed once with 1 × PBS, fixed for 15 min in 4% paraformaldehyde (Thermo Fisher Scientific) in PBS, and then washed once with distilled water. Cells were then stained for 20 min in a 1% crystal violet solution in water (Sigma‐Aldrich). Cells were washed three times in water and dishes were dried overnight. Plate images were obtained via the Epson Perfection V800 Photo scanner.
9
Morphometric Analysis of Lotus corniculatus
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A straightedge was used to measure the plant height. The aboveground and belowground sections of the plant were separated and the fresh weights were measured before these parts were placed in an oven at 105°C for 30 min and dried to a constant weight at 65°C to determine the dry weights of the aboveground and belowground portions. The molybdenum blue technique was used to measure the total P concentration of L. corniculatus (Chapman and Pratt, 1962 (link)). Fresh leaves and roots were scanned with an Epson Perfection V800 Photo scanner, and the resulting images were analyzed with WinFOLIA Pro 2015 and WinRHIZO Pro 2015 software (Regent Instructions, Canada Inc.) to obtain leaf indicators (surface area, length, width, length/width and perimeter) and root indicators (total length, total surface area, diameter, volume, tip number and hair number), respectively.
10
Measuring Plant Water and Growth Response
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The relative water content (RWC) of leaves = (FWL − DW)/(TW − DW) × 100, where FWL represents the fresh weight of the leaf sample, TW represents the weight of the leaf after it was immersed in distilled water in the dark for 24 h and DW represents the dry weight after heating at 65°C for 24 h.
The growth rate of plant height = (before treatment plant height—after treatment plant height)/12. The absolute plant height (the vertical height from the ground to the top of the plant) was measured before and after treatment. The RWC and growth rate were measured for 10 plants in each treatment.
Leaf samples were imaged with an Epson Perfection V800 Photo Scanner. The leaf parameters, including leaf width, leaf length, and leaf area, were analysed using WinFOLIA software (Regent Instruments, Inc., Canada). The fresh seedlings were divided into aerial parts and root systems, and then, both aerial parts and roots were dried at 105°C for 1 h and then at 80°C for 72 h followed by weighing.
The growth rate of plant height = (before treatment plant height—after treatment plant height)/12. The absolute plant height (the vertical height from the ground to the top of the plant) was measured before and after treatment. The RWC and growth rate were measured for 10 plants in each treatment.
Leaf samples were imaged with an Epson Perfection V800 Photo Scanner. The leaf parameters, including leaf width, leaf length, and leaf area, were analysed using WinFOLIA software (Regent Instruments, Inc., Canada). The fresh seedlings were divided into aerial parts and root systems, and then, both aerial parts and roots were dried at 105°C for 1 h and then at 80°C for 72 h followed by weighing.
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