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Ab3786

Manufactured by Abcam
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Sourced in United Kingdom

AB3786 is a polyclonal antibody that detects the Amyloid beta A4 precursor protein (APP) in various species including human, mouse and rat. It is suitable for applications such as Western blotting and immunohistochemistry.

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Lab products found in correlation

D3571, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 goat anti rabbit antibody, by Thermo Fisher Scientific (1 mentions) Ab28364, by Abcam (1 mentions) Ab16667, by Merck Group (1 mentions) Ab75869, by Abcam (1 mentions) Ab68459, by Abcam (1 mentions) Ab52635, by BioLegend (1 mentions) Gfp 1020, by Abcam (1 mentions) Af2018, by Abcam (1 mentions) Sc 393002, by Santa Cruz Biotechnology (1 mentions) Sc 365992, by R&D Systems (1 mentions)

4 protocols using ab3786

1

Immunofluorescence Staining of Lung Cells

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Approximately 105 cells were resuspended in PBS and spun onto VWR SuperFrost Plus microscope slides (Radnor, PA), fixed in 3% paraformaldehyde, and incubated with either pro-SPC (Millipore ab3786, Burlington, MA) or CCSP separately (Abcam 40873, Cambridge, MA) followed by washing and incubation with the secondary Alexafluor 488 goat anti-rabbit antibody (Life Technologies A11008, Carlsbad, CA), re-washed, and incubated with DAPI (Molecular Probes D3571, Carlsbad, CA) prior to cover slipping. Quantitation was performed manually.
Cole N., Krockenberger M., Bao S., Beagley K.W., Husband A.J, & Willcox M. (2001). Effects of Exogenous Interleukin-6 during Pseudomonas aeruginosa Corneal Infection. Infection and Immunity, 69(6), 4116-4119.
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2

Lung Tissue Analysis for Pathology

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Mice were sacrificed by carbon dioxide asphyxiation. Lungs were dissected under a fluorescent stereoscope, fixed in 4% formaldehyde or 10% formalin overnight, embedded in paraffin, sectioned at 6 μm, and stained with hematoxylin and eosin (HE) for pathology. For tumor size quantification, images were taken tiling the whole lobe, merged into a single lobe, and tumors were manually outlined as region of interest (ROI) and were subsequently quantified using ImageJ (Schneider et al., 2012 (link)). Sections were de-waxed, rehydrated, and stained using standard immunohistochemistry (IHC) protocols as previously described (Chen et al., 2014 (link)). The following antibodies were used for IHC: anti-Ki67 (abcam ab16667, 1:100), anti-CCSP (Millipore, 1:500), anti-pSPC (Millipore AB3786, 1:500) and anti-CD31 (abcam, ab28364, 1:50). IHC was quantified in 10 randomly chosen low-magnification fields per lobe with 3 mice per group using ImageJ (Schneider et al., 2012 (link)).
Platt R.J., Chen S., Zhou Y., Yim M.J., Swiech L., Kempton H.R., Dahlman J.E., Parnas O., Eisenhaure T.M., Jovanovic M., Graham D.B., Jhunjhunwala S., Xavier R.J., Langer R., Anderson D.G., Hacohen N., Regev A., Feng G., Sharp P.A, & Zhang F. (2014). CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Cell, 159(2), 440-455.
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3

Immunohistochemical Analysis of IPF Lung Sections

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Immunohistochemistry (IHC) of the lung sections was performed as previously described in detail20 . The primary antibodies used for IHC are listed as follows: proSP-C rabbit polyclonal antibody (1:750, Merck Millipore, AB3786), rabbit monoclonal antibody for human cytokeratin-5 (KRT5) (1:200, Abcam, Cambridge, United Kingdom, ab75869), rabbit monoclonal antibody for human cytokeratin-7 (KRT7) (1:200, Abcam, ab68459), HOPX mouse monoclonal antibody (1:50, Santa Cruz Inc., sc-398703), and mouse monoclonal antibody for human Ki67 (1:50, Dianova, DIA-670-P05). Immunostained lung sections were scanned with a scanning device (Nano-Zoomer, Hamamatsu), and examined histopathologically using the ‘NDP.view2 software’ at 200×, 400× and 800× original magnification. IHC for mentioned antibodies was undertaken in 12 IPF- and 5 control-donor lung samples.
Ota C., Ng-Blichfeldt J.P., Korfei M., Alsafadi H.N., Lehmann M., Skronska-Wasek W., M. De Santis M., Guenther A., Wagner D.E, & Königshoff M. (2018). Dynamic expression of HOPX in alveolar epithelial cells reflects injury and repair during the progression of pulmonary fibrosis. Scientific Reports, 8, 12983.
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4

Immunohistochemical Profiling of Lung Cells

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Mice were euthanized by inhalation of CO2, and the lungs were perfused with PBS through the right ventricle. The lungs were then inflated with 2% PFA at a constant pressure of 25 cm H2O and were fixed overnight at 4 °C. Tissues were then dehydrated, embedded in paraffin, and sectioned. For immunohistochemistry, the following antibodies were used: GFP (chicken, Aves, GFP-1020, 1:200), Nkx2-1 (rabbit, Abcam, ab76013, 1:100), Sox2 (goat, R&D, AF2018, 1:50), Sox9 (rabbit, Abcam, ab185966, 1:100), Scgb1a1 (mouse, Santa Cruz, sc-365992, 1:100), SCGB1A1 (rat, R&D, MAB4218, 1:40), Scgb3a2 (mouse, Novus, H00117156-M01, 1:100), β-Tubulin IV (mouse, Biogenex, MU178-UC, 1:20), p63 (rabbit, Santa Cruz, sc-8343, 1:100), β-catenin (mouse, BD Biosciences, 610154, 1:100), Sftpc (rabbit, Millipore, AB3786, 1:200), SFTPC (rabbit, Abcam, ab90716, 1:100), Lamp3 (rat, Novus, 1010E1.01, 1:100), Hopx (mouse, Santa Cruz, sc-398703, 1:100), Krt5 (rabbit, Abcam, ab52635, 1:500), Krt5 (chicken, BioLegend, 905901, 1:100), Krt8 (rat, DSHB, TROMA-1, 1:100), and Krt17 (mouse, Santa Cruz, sc-393002, 1:200).
Shiraishi K., Morley M.P., Jones D.L., Zhao G., Weiner A.I., Basil M.C., Cantu E., Ferguson L.T., Oyster M., Babu A., Ying Y., Zhou S., Li S., Vaughan A.E, & Morrisey E.E. (2024). Airway epithelial cell identity and plasticity are constrained by Sox2 during lung homeostasis, tissue regeneration, and in human disease. NPJ Regenerative Medicine, 9, 2.
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