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2 protocols using deferoxamine mesylate

1

High-throughput Drug Screening in HepG2 Cells

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HepG2 or HB214 (1500 cells/well) was seeded on a 384 well plate in 30 μl. Drugs were added after 24 h. Cell Titer Glo (CTG) viability assay was conducted after 72 h of drug treatment. Briefly, CellTiter-Glo 2.0 (#G9243 Promega) was added to each well in a 1:1 v/v ratio. Plates were then covered to keep from light and incubated at RT on an orbital shaker at 150 RPM for 30 mins. After the incubation, plate was read on a synergy H4 plate reader for luminescence. Dose effect curves for each drug were calculated using Prism software, version 9 (GraphPad). For drug combinations, responses were analyzed using SynergyFinder2.050 (link). Drugs used include cisplatin (#479036-5 G, Sigma Aldrich), gemcitabine (#AC456890010, Fisher Scientific), vincristine (#AAJ60907MA, Fisher Scientific), triapine (#50-136-4826, Fisher Scientific), MK1775 (#M4102, LKT laboratories, Saint Paul, Minnesota), doxorubicin (#BP25161, Fisher Scientific), sorafenib (#NC0749948, Fisher Scientific), SN-38 (#S4908-50MG, Selleck Chemicals, Harris County, Texas), deferoxamine mesylate (#AC461770010, Acros Organics, Geel, Belgium), KU60019 (#S1570, Selleck Chemicals). All concentrations were seeded in triplicate and the experiment was repeated three times. Significance was determined using the Extra Sum of Squares f test.
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2

Synthesis and Characterization of Multifunctional Theranostic Nanoparticles

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2-methacryloyloxyethyl phosphorylcholine (MPC, 97%), L-serine (≥99.5%), sodium phosphotungstate hydrate, sodium carbonate (≥99.5%), TWEEN 80, 4-cyano-4-(phenylcarbonothioylthio) pentanoic acid (CPD, 97%), and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldric. HPLC-grade tetrahydrofuran and chloroform, acetone, deuterium oxide, and deferoxamine mesylate (DFO, 95%) were purchased from Acros Organics. 4,4’-azobis (4-cyanovaleric acid) (ACVA, 98%), curcumin (95%), acryloyl chloride (55%), and iron (III) chloride hexahydrate were purchased from Alfa Aesar. Nanosep centrifugal devices with Omega membrane (3K MWCO) were purchased from Pall Corporation. Dulbecco’s Modified Eagle’s Medium (DMEM), fetal bovine serum (FBS), and (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) (MTT) assay kits were purchased from Thermo Fisher Scientific. Dialysis membranes (8K MWCO) were purchased from Spectrum Laboratories, Inc. Copper mesh was purchased from Ted Pella. Copper sulfate was purchased from Showa Co., Ltd. MDA-MB-231 breast cancer cells were purchased from the Bioresource Collection and Research Center in Taiwan. Deionized water with a minimum resistivity of 18.0 MΩ·m was used in experiments.
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