Anti ddx60

Manufactured by Abcam

Anti-DDX60 is a primary antibody that recognizes the DDX60 protein. DDX60 is a RNA helicase involved in the innate immune response to viral infections. The antibody can be used to detect the expression of DDX60 in various samples through techniques such as Western blotting.

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Lab products found in correlation

Anti actin, by Merck Group (1 mentions) Anti traf3, by Santa Cruz Biotechnology (1 mentions) Anti snrnp200, by Merck Group (1 mentions) Anti rela, by Santa Cruz Biotechnology (1 mentions) Anti nhp2l1, by Abcam (1 mentions) Anti ddx3x, by Fortis Life Sciences (1 mentions) Anti irf3, by Santa Cruz Biotechnology (1 mentions) Hrp conjugated secondary antibody, by Bio-Rad (1 mentions) Protease inhibitor cocktail, by Roche (1 mentions) Western lighting chemiluminescence reagent plus, by PerkinElmer (1 mentions) Anti p65, by Proteintech (1 mentions) Anti phospho ikkα β, by Cell Signaling Technology (1 mentions) Anti traf3, by Proteintech (1 mentions) Normal rabbit igg, by Cell Signaling Technology (1 mentions) Phospho p38, by Cell Signaling Technology (1 mentions) Normal mouse igg, by Cell Signaling Technology (1 mentions) Anti ythdf1, by Proteintech (1 mentions) Anti myd88, by Proteintech (1 mentions) Anti phosphor iκbα, by Cell Signaling Technology (1 mentions) Anti phosphor p65, by Cell Signaling Technology (1 mentions)

2 protocols using anti ddx60

Comprehensive Cell Lysis and Immunoblotting

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Cells were washed twice with ice-cold phosphate-buffered saline (PBS; Wisent), harvested and lysed in 10mM Tris-HCl, 100mM NaCl, 0.5% Triton X-100, pH7.6 with EDTA-free Protease Inhibitor Cocktail (Roche). Cell lysates were clarified by centrifugation at 13,000 g for 20 min at 4°C and subjected to sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE). Western blot analysis was performed using mouse anti-PHF5A (Abnova), anti-IRF3 (Santa Cruz), anti-TRAF3 (Santa Cruz), anti-RIG-I (Alexis Biochemicals), anti-ACTIN (Chemicon International), anti-TBK1 (Imgenex and Santa Cruz), anti-IKBKE (Santa Cruz), anti-TUBULIN (ICN), anti-GAPDH (RDI) and rabbit anti-SNRNP200 (Sigma-Aldrich), anti-SF3A1 (Santa Cruz), anti-RELA (Santa Cruz), anti-NHP2L1 (Abcam), anti-DDX3X (Bethyl), anti-DDX60 (Abcam), TRIF (Cell Signaling), anti-ISG56 (Novus Biologicals), anti-MDA5 (Alexis Biochemicals), anti-MAVS (Alexis Biochemicals), anti-IKBKE (eBioscience), STAT1 (ABCAM), STAT1 tyr701 (ABCAM), IFNAR1 (Santa Cruz) and anti-IRF3-P-ser386 (Abcam). HRP-conjugated secondary antibodies were from Bio-Rad. The chemiluminescence reaction was performed using the Western Lighting Chemiluminescence Reagent Plus (PerkinElmer).

Tremblay N., Baril M., Chatel-Chaix L., Es-Saad S., Park A.Y., Koenekoop R.K, & Lamarre D. (2016). Spliceosome SNRNP200 Promotes Viral RNA Sensing and IRF3 Activation of Antiviral Response. PLoS Pathogens, 12(7), e1005772.

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Antibody Validation for m6A Studies

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The following antibodies were used in this study: anti-m⁶A (Synaptic Systems, 202003 for m⁶A-seq; Abcam, ab208577 for m⁶A-IP qPCR), anti-YTHDF1 (Proteintech, 66745–1-lg), anti-TRAF6 (Abcam, ab181622), anti-TRAF3 (Proteintech, 18099-1-AP), anti-β-actin (HuaAn, R1207–1), anti-Flag (Sigma-Aldrich, F3165), anti-MYC (Abcam, ab32), anti-DDX60 (Abcam, ab139807), anti-MyD88 (Proteintech, 23230-1-AP), anti-phosphor-p65 (Cell Signaling Technology, 3033), anti-phosphor-IκBα (Cell Signaling Technology, 2859), anti-TRIF (Proteintech, 23288-1-AP), anti-p65 (Proteintech, 10745-1-AP), anti-IκBα (Proteintech, 10268-1-AP), anti-BCLAF1 (Proteintech, 26809-1-AP), anti-THRAP3 (Proteintech, 19744-1-AP), anti-TRAF3 (Proteintech, 18099-1-AP), anti-phospho-IKKα/β (Cell Signaling Technology, 2078), anti-IKKα (HuaAn, ER30911), anti-IKKβ (HuaAn, ER1706-13), anti-p38 (HuaAn, ET1602-26), phospho-p38 (Cell Signaling Technology, 9211), anti-phospho-JNK (HuaAn, RT1488), anti-JNK (HuaAn, RT1550), anti-ERK1/2 (Cell Signaling Technology, 9102), phospho-ERK1/2 (HuaAn, ET1610–13), normal rabbit IgG (Cell Signaling Technology, 2729) and normal mouse IgG (Cell Signaling Technology, 5946).

Zong X., Xiao X., Shen B., Jiang Q., Wang H., Lu Z., Wang F., Jin M., Min J., Wang F, & Wang Y. (2021). The N6-methyladenosine RNA-binding protein YTHDF1 modulates the translation of TRAF6 to mediate the intestinal immune response. Nucleic Acids Research, 49(10), 5537-5552.

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