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Western blot gels

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Western blot gels are laboratory equipment used to separate and analyze proteins in a sample. They function by applying an electric current to the gel, causing the proteins to migrate based on their molecular weight. This allows researchers to identify and quantify specific proteins within a complex mixture.

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Lab products found in correlation

Odyssey blocking buffer, by LI COR (4 mentions) Protein sample loading buffer, by LI COR (4 mentions) Pampkα1 α2, by Cell Signaling Technology (3 mentions) Goat anti rabbit ir 800 and goat anti mouse ir 680 antibodies, by LI COR (3 mentions) Primary antibodies for ampkα1 α2, by Cell Signaling Technology (2 mentions) Metformin, by Enzo Life Sciences (2 mentions) Nupage lds sample buffer 4, by Thermo Fisher Scientific (2 mentions) β actin, by Santa Cruz Biotechnology (2 mentions) Nupage lds sample buffer 4x, by Thermo Fisher Scientific (1 mentions) Primary antibodies for lkb1, by Santa Cruz Biotechnology (1 mentions) Lc3b 1, by Cell Signaling Technology (1 mentions) Lc3b 2, by Cell Signaling Technology (1 mentions) Ampkα1 α2, by Cell Signaling Technology (1 mentions) Aicar, by LC Laboratories (1 mentions) Nupage lds sample buffer, by Thermo Fisher Scientific (1 mentions)

4 protocols using western blot gels

1

Metformin Regulates AMPK and SIRT1

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Metformin was obtained from Enzo Life Sciences (Farmingdale, NY) Primary antibodies for AMPKα1/α2 and pAMPKα1/α2 were obtained from Cell Signaling Technology (Danvers, MA). Primary antibodies for SIRT1, GAPDH and PGC1-α were obtained from Abcam (Cambridge, MA). The Odyssey blocking buffer, LI-COR goat anti-rabbit IR-800 and goat anti-mouse IR-680 antibodies, and the 4X Protein Sample Loading Buffer were obtained from LI-COR Biotechnology (Lincoln, NE). The NuPAGE® LDS Sample Buffer (4X), and Western blot gels were purchased from Life Technologies (Grand Island, NY). All other chemicals were obtained from Sigma-Aldrich (St. Louis, MO).
Matsiukevich D., Piraino G., Lahni P., Hake P.W., Wolfe V., O’Connor M., James J, & Zingarelli B. (2017). METFORMIN AMELIORATES GENDER- AND AGE-DEPENDENT HEMODYNAMIC INSTABILITY AND MYOCARDIAL INJURY IN MURINE HEMORRHAGIC SHOCK. Biochimica et biophysica acta, 1863(10 Pt B), 2680-2691.
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2

Metformin Regulation of AMPK Signaling

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Metformin was obtained from Enzo Life Sciences (Farmingdale, NY). Primary antibodies for AMPKα1/α2 and pAMPKα1/α2 were obtained from Cell Signaling Technology (Danvers, MA). Primary antibodies for LKB1, pLKB1 and β-actin were obtained from Santa Cruz Biotechnology (Dallas, TX). Primary antibody for PGC1-α was obtained from Abcam (Cambridge, MA). The Odyssey blocking buffer, LI-COR goat anti-rabbit IR-800 and goat anti-mouse IR-680 antibodies, and the 4× Protein Sample Loading Buffer were obtained from LI-COR Biotechnology (Lincoln, NE). The Multiplex Luminex Array kit, the NuPAGE® LDS Sample Buffer (4×), and Western blot gels were purchased from Life Technologies (Grand Island, NY). All other chemicals were obtained from Sigma-Aldrich (St. Louis, MO).
Kim P., Piraino G., O’Connor M., Hake P.W., Wolfe V., Lahni P, & Zingarelli B. (2018). Metformin exerts beneficial effects in hemorrhagic shock in an AMPKα1-independent manner. Shock (Augusta, Ga.), 49(3), 277-287.
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3

AICAR Signaling Pathway Analysis

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The AMP analog, AICAR, was obtained from LC Laboratories (Woburn, MA). Primary antibodies for AMPKα1/α2, pAMPKα1/α2, and LC3B-I and LC3B-II were obtained from Cell Signaling Technology (Danvers, MA). Primary antibodies for GAPDH and for PGC1-α were obtained from Abcam (Cambridge, MA). The Odyssey blocking buffer, LI-COR goat anti-rabbit IR-800 and goat anti-mouse IR-680 antibodies, and the 4× Protein Sample Loading Buffer were obtained from LI-COR Biotechnology (Lincoln, NE). The NuPAGE® LDS Sample Buffer (4×), and Western blot gels were purchased from Life Technologies (Grand Island, NY). All other chemicals were obtained from Sigma-Aldrich (St. Louis, MO).
Matsiukevich D., Piraino G., Klingbeil L.R., Hake P.W., Wolfe V., O’Connor M, & Zingarelli B. (2017). THE AMPK ACTIVATOR AICAR AMELIORATES AGE-DEPENDENT MYOCARDIAL INJURY IN MURINE HEMORRHAGIC SHOCK. Shock (Augusta, Ga.), 47(1), 70-78.
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4

Humanin-G Signaling Pathway Analysis

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Humanin-G ([Gly14]-Humanin) was obtained from AnaSpec (Fremont, CA). Primary antibodies for STAT3, pSTAT3(Tyr705), pSTAT3(Ser727), and VDAC1 anti-rabbit were obtained from Cell Signaling Technology (Danvers, MA). Primary antibodies for GRIM19, β-actin, and VDAC1 anti-mouse were obtained from Santa Cruz Biotechnology (Dallas, TX). The Odyssey blocking buffer, LI-COR goat anti-rabbit IR-800, goat antimouse IR-680 antibodies, and the 4X Protein Sample Loading Buffer were obtained from LI-COR Biotechnology (Lincoln, NE). The IBind Western System solution kit was purchased from Thermo Fischer Scientific (Waltham, MA). The NuPAGE LDS Sample Buffer, and Western blot gels were purchased from Life Technologies (Grand Island, NY). All other chemicals were obtained from Sigma-Aldrich (St. Louis, MO).
Wagner M.L., Ammann A., Piraino G., Wolfe V., O'Connor M., Lahni P., Ziady A, & Zingarelli B. (2023). PROTECTIVE EFFECTS OF HUMANIN-G IN HEMORRHAGIC SHOCK IN FEMALE MICE VIA AMPKα1-INDEPENDENT MECHANISMS. Shock (Augusta, Ga.), 60(1).
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