The human MM OPM-2 and NCI-H929 cell lines (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany), and OPM-2-lucifease+ (luc+) cells [21 (link)] were cultured in RPMI 1640 (Wako Pure Chemical Industries, Osaka, Japan) containing 10% heat-inactivated, exosome-depleted fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA) and 1% penicillin-streptomycin (PC/SM; Wako Pure Chemical Industries). The human cervical cancer line, HeLa cell (American Type Culture Collection, Manassas, VA, USA), was cultured in Dulbecco’s Modified Eagle’s medium (DMEM) (Wako Pure Chemical Industries) containing 10% FBS and 1% PC/SM. Depletion of exosomes from FBS was performed as previously described [17 (link)]. All cell lines were maintained at 37 °C in a fully humidified atmosphere of 20% O2, 5% CO2, and 75% N2.
To confirm the function of Ab-conjugated complexes, we established HeLa-luc cells. Approximately 1 × 104 HeLa cells were transfected with 25 ng of the pGL4.13[luc2/SV40] vector (luc2 reporter gene 499–2151) (Promega, Madison, WI, USA) using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA, USA), according to the manufacturer’s instructions.
To confirm the function of Ab-conjugated complexes, we established HeLa-luc cells. Approximately 1 × 104 HeLa cells were transfected with 25 ng of the pGL4.13[luc2/SV40] vector (luc2 reporter gene 499–2151) (Promega, Madison, WI, USA) using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA, USA), according to the manufacturer’s instructions.