Antibodies used in this study are as follows: SNX1 [clone 51; 611482; BD Bioscience; immunofluorescence (IF) 1:200], GLUT1 (ab40084; Abcam; IF 1:200), Golgin97 (clone CDF4; A-21270; Thermo Fisher Scientific; IF 1:400), VPS26 (ab23892; Abcam; IF 1:200), VPS35 (ab10099; Abcam; IF 1:200), VPS35 [ab97545; Abcam; IF 1:200), VPS35 [ab157220; Abcam; western blotting (WB) 1:1000], VPS29 (ab98929; Abcam; WB 1:200), FAM21 (gift from Daniel D. Billadeau, Mayo Clinic, Rochester, MN; IF 1:400), EEA1 (N-19; sc-6415; Santa Cruz Biotechnology; IF 1:200), TGN46 (AHP500G; Bio-Rad Laboratories; IF 1:200), anti-Myc (gift from Harry Mellor, The University of Bristol, UK; IF 1:500), LAMP1 (DSHB Hybridoma Product; H4A3; deposited to the DSHB by August, J.T./Hildreth, J.E.K.; IF 1:500), LAMP1 (ab24170; Abcam; IF 1:200), mouse EEA1 (610457; BD Bioscience; IF 1:200), CI-MPR (ab124767; Abcam; WB 1:1000, IF 1:200), Itgβ1 (TS2/16; IF 1:200), SNX6 (Clone D-5; 365965; Santa Cruz Biotechnology; WB 1:500), PMP70 (PA1-650; Invitrogen; IF 1:200), WASH1 (gift from Daniel D. Billadeau; IF 1:400), C16orf62 (PA5-28553; Pierce; IF 1:200), sortilin (ab16640; Abcam; WB 1:1000), cathepsin D (21327-1-AP, Proteintech; WB 1:1000), SNX5 (ab180520; Abcam; WB 1:500) and β-actin (A1978; Sigma-Aldrich; WB 1:2000).
Golgin97 clone cdf4
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
Golgin97 (clone CDF4) is a protein that is commonly used as a marker for the Golgi apparatus in cells. It is a member of the golgin family of proteins, which are structural components of the Golgi complex. Golgin97 is typically used in immunofluorescence and Western blotting applications to visualize and study the Golgi apparatus in various cell types and research contexts.
Automatically generated - may contain errors
Lab products found in correlation
Ab40084, by Abcam (1 mentions)
Ab16640, by Abcam (1 mentions)
Ab23892, by Abcam (1 mentions)
Ab97545, by Abcam (1 mentions)
Ab98929, by Abcam (1 mentions)
Ab180520, by Abcam (1 mentions)
Ab10099, by Abcam (1 mentions)
Ab24170, by Abcam (1 mentions)
Ab124767, by Abcam (1 mentions)
Ab157220, by Abcam (1 mentions)
Ahp500g, by Bio-Rad (1 mentions)
Pa1 650, by Thermo Fisher Scientific (1 mentions)
Tgn46, by Bio-Rad (1 mentions)
Snx1 clone 51 snx1, by BD (1 mentions)
Snx2 clone 13 snx2, by BD (1 mentions)
Itga5, by BD (1 mentions)
Galnt2, by Abcam (1 mentions)
Vps35, by Abcam (1 mentions)
Calnexin, by Abcam (1 mentions)
β actin, by Merck Group (1 mentions)
2 protocols using golgin97 clone cdf4
1
Antibody Panel for Endosomal Trafficking
2
Antibody Characterization for Cell Biology
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Antibodies used in this study were as follows: GFP (clones 7.1 and 13.1; 11814460001; Roche) (1:2,000 for Western blot (WB), 1:400 for immunofluorescence (IF)), SNX1 (clone 51/SNX1; 611482; BD) (1:1,000 for WB, 1:200 for IF), SNX2 (clone 13/SNX2; 5345661; BD) (1:1,000 for WB, 1:200 for IF), SNX6 (clone d-5,365965; Santa Cruz Biotechnology, Inc.) (1:1,000 for WB, 1:200 for IF), SNX5 (clone EPR14358; ab180520; Abcam) (WB 1:500), β-actin (A1978; Sigma-Aldrich) (1:2,000 for WB), ITGA5 (610633, BD) (1:1,000 for WB); VPS35 (97545; Abcam) (IF 1:200), GFP (GTX20290; GeneTex) (WB 1:2,000), EEA1 (C45B10; Cell Signaling Technologies) (IF 1:200), LAMP1 (clone H4A3, AB2296838; DSHB) (IF 1:200) Cathepsin D (21327-1-AP; Proteintech) (WB 1:1,000), Calnexin (ab22595; Abcam) (WB 1:1,000), TGN46 (AHP500G, Bio-Rad) (IF 1:400), Golgin-97 (clone CDF4; A-21270; Thermo Fischer) (IF 1:200), HA tag (66006-1-Ig, Proteintech) (WB 1:1,000), NCAD (clone 13A0; 14215; Cell Signaling) (WB 1:1,000), Tom20 (clone 29; 612278; BD Biosciences), GALNT2 (ab102650; Abcam) (WB 1:1,000), NRP1 (clone EPR3113; ab81321; Abcam) (WB 1:1,000, IF 1:200), NRP1 b1b2 domain (23 (link), 24 (link)) (clone 3E7.1) (IF 1:200), Tubulin (ab6046; Abcam) (WB 1:2,000), SARS-CoV-2 spike (40592-T62, Sino Biologicals) (WB 1:1,000) SARS Nucleocapsid (200-401-A50, Rockland) (IF 1:2,000).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!