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Douset elisa system

Manufactured by R&D Systems
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The Douset ELISA system is a laboratory instrument designed for enzyme-linked immunosorbent assay (ELISA) testing. It is used to quantify the presence and concentration of target analytes in samples by employing specific antibody-antigen interactions and colorimetric detection.

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DouSet ELISA ELISA systems ELISAs

3 protocols using douset elisa system

1

IFN-γ Quantification in Activated Lymphoblastoid Cells

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Human IFN-γ was detected using DouSet ELISA system (R&D System) as per manufactures instructions. EBV-immortalized lymphoblastoid cell lines generated from Type1 diabetes patients were activated with 100 ng/ml PMA plus 1 μg/ml ionomycin (PMA+I). Culture supernatants were collected after 48 hrs of induction. Supernatants were collected at indicated time points and IFN-γ was measured by DouSet ELISA system.
Pandey R., Bakay M., Hain H.S., Strenkowski B., Elsaqa B.Z., Roizen J.D., Kushner J.A., Orange J.S, & Hakonarson H. (2018). CLEC16A regulates splenocyte and NK cell function in part through MEK signaling. PLoS ONE, 13(9), e0203952.
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2

Cytokine Production Quantification

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Collection of supernatants from splenic cell and peritoneal cell proliferation cultures were used to determine cytokine production by ELISA. The concentrations of cytokines of IL-6, TNF-α, IL-2, and IFN-γ were determined by using the Douset ELISA system method according to manufacturer’s instructions (R&D systems, Minneapolis, MN).
Belay T., Fu C.L, & Woart A. (2015). Active Hexose Correlated Compound Activates Immune Function to Decrease Chlamydia trachomatis Shedding in a Murine Stress Model. Journal of nutritional medicine and diet care, 1(1), JNMDC-1-006.
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3

Cytokine Production Quantification

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Collection of supernatants from splenic cell and peritoneal cell proliferation cultures were used to determine cytokine production by ELISA. The concentrations of cytokines of IL-6, TNF-α, IL-2, and IFN-γ were determined by using the Douset ELISA system method according to manufacturer’s instructions (R&D systems, Minneapolis, MN).
Belay T., Fu C.L, & Woart A. (2015). Active Hexose Correlated Compound Activates Immune Function to Decrease Chlamydia trachomatis Shedding in a Murine Stress Model. Journal of nutritional medicine and diet care, 1(1), JNMDC-1-006.
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