Radioimmunoprecipitation assay buffer ripa

Monoclonal antibody against β-myosin heavy chain (β-MHC; catalogue no. ab55152) was from Abcam (Cambridge, MA, USA). Monoclonal antibody anti-cardiac troponin T (cTnT; catalogue no. LI4C00702) was purchased from Shanghai Linc-Bio Science Co. Ltd. (Shanghai, China; http://lzhenyul.biogo.net/sell/typeid-39.shtml), Polyclonal antibody anti-connexin-43 (CX-43; catalogue no. RLT1044) was purchased from Ruiying Biological (Suzhou, Jiangsu, China; http://www.rlgene.com/). Polyclonal antibody anti-N-cadherin (N-cad; catalogue no. YT745) were purchased from Baiaolaibo Company (Beijing, China; http://www.bjbalb.com/). Anti-AKT (catalogue no. 4691) and anti-p-AKT (catalogue no. 13038) antibodies for western blot analysis were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). GAPDH monoclonal antibodies (catalogue no. AG019) and bicinchoninic acid assay (BCA) kits (catalogue no. P0011) were obtained from Beyotime Institute of Biotechnology (Haimen, China). Ultrapure RNA kit (catalogue no. CW0581S) and SuperRT cDNA Synthesis kit (catalogue no. CW0741S) were purchased from CW Biotech (Beijing, China). Radioimmunoprecipitation assay buffer (RIPA, catalogue no. R0010) was purchased from Solarbio Life Science (Beijing, China).

Zein, sodium caseinate, sodium alginate, curcumin (>98%), and Trolox (>97%) were obtained from Acros Organics (New Jersey, USA). 1, 1-Diphenyl-2-picrylhydrazyl free radical (DPPH·, >97%) and 2, 2′-azino-bis (3- ethyl-benzothiazoline-6-sulfonic acid) diammonium salt (ABTS, >98%) were obtained from TCI (Tokyo, Japan). Bile salts, pancreatin (from porcine pancreas, 8 × USP specifications), and pepsin (from porcine gastric mucosa, 535 U/mg) were obtained from Sigma-Aldrich (St Louis, MO, USA). Ascorbic acid (99%) was obtained from InnoChem Science & Technology (Beijing, China). A radio-immunoprecipitation assay buffer (RIPA) was obtained from Solarbio (Beijing, China). Cell counting kit-8 (CCK-8) and Dulbecco’s Modified Eagle Medium (DMEM) were obtained from Gibco (Shanghai, China). Fetal bovine serum (FBS), penicillin-streptomycin solution, and 0.25% trypsin-0.04% EDTA were purchased from Hyclone (South Logan, Utah, USA). 2, 7-dichlorofluoroescin diacetate (DCF-DA) was purchased from MedChemExpress, USA. Test kits of superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) were purchased from Jiancheng Bioengineering Institute (Nanjing, China).