Corn oil

Eight-week-old C57BL/6 mice were purchased from SPF Biotechnology Co., Ltd. (Beijing, China) and bred in a specific pathogen-free facility at the Experimental Animal Center of Nanfang Hospital. Food and water were provided ad libitum under a 12/12 h light/dark cycle. To induce acute liver injury, mice were administered one intraperitoneal injection of 10 mL/kg of 5% CCl 4 (Rhawn Co., Ltd., Shanghai, China) dissolved in corn oil, and the control group was administered one intraperitoneal injection of just corn oil (Macklin, Shanghai, China). Mice were treated with metabolites or phosphate buffered saline (PBS) at 2 h after injection of CCl 4 . Several available differential metabolites were divided into water-soluble and non-water-soluble metabolites. For the non-water-soluble metabolites, mice with liver injury were administered 100 mg/kg of the corresponding metabolites by oral gavage. For water-soluble metabolites, mice with liver injury were intraperitoneally injected with 100 mg/kg of the corresponding metabolites. Blood was collected 24 and 48 h after CCl 4 injection. All mice were sacrificed 72 h after injection, and blood and liver samples were collected. The protocols for animal experiments were approved by the Institutional Animal Ethics Committee of the Experimental Animal Center of Nanfang Hospital.