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Rsv a and b isolates

Manufactured by Abcam

Abcam's RSV A and B isolates are purified samples of the respiratory syncytial virus (RSV) subtypes A and B. These isolates can be used for research purposes, such as the development and evaluation of diagnostic tests or the study of viral characteristics and behaviors.

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2 protocols using rsv a and b isolates

1

Quantifying Viral Titers and Lung Infection

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Infectious virus titers were determined by plaque assays. Lungs of infected mice were excised and homogenized using a mechanical homogenizer (Kinematica, Bohemia, NY, USA). The viral titers in the homogenates were quantified by plaque assay on epithelial cells using methyl cellulose overlay media (R&D Systems) and staining with 0.5 mg/ml thiazolyl blue tetrazolium bromide (MTT; Sigma Aldrich) solution in PBS for 3 hours at 37°C. Paraffin-embedded lung samples were sectioned at 5 μm, and stained with polyclonal anti-RSV antibody that recognizes RSV antigen from RSV A and B isolates (Abcam) and antibodies using an immunoperoxidase technique (Vectastain ABC Elite kit, Vector Laboratories, Burlingame, CA) to determine areas of lung infected.
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2

Quantifying Viral Titers and Lung Infection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Infectious virus titers were determined by plaque assays. Lungs of infected mice were excised and homogenized using a mechanical homogenizer (Kinematica, Bohemia, NY, USA). The viral titers in the homogenates were quantified by plaque assay on epithelial cells using methyl cellulose overlay media (R&D Systems) and staining with 0.5 mg/ml thiazolyl blue tetrazolium bromide (MTT; Sigma Aldrich) solution in PBS for 3 hours at 37°C. Paraffin-embedded lung samples were sectioned at 5 μm, and stained with polyclonal anti-RSV antibody that recognizes RSV antigen from RSV A and B isolates (Abcam) and antibodies using an immunoperoxidase technique (Vectastain ABC Elite kit, Vector Laboratories, Burlingame, CA) to determine areas of lung infected.
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