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Rsv a and b isolates

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Abcam's RSV A and B isolates are purified samples of the respiratory syncytial virus (RSV) subtypes A and B. These isolates can be used for research purposes, such as the development and evaluation of diagnostic tests or the study of viral characteristics and behaviors.

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Lab products found in correlation

Methyl cellulose overlay media, by R&D Systems (2 mentions) Vectastain elite abc kit, by Vector Laboratories (2 mentions) Mechanical homogenizer, by Kinematica (2 mentions)

2 protocols using rsv a and b isolates

1

Quantifying Viral Titers and Lung Infection

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Infectious virus titers were determined by plaque assays. Lungs of infected mice were excised and homogenized using a mechanical homogenizer (Kinematica, Bohemia, NY, USA). The viral titers in the homogenates were quantified by plaque assay on epithelial cells using methyl cellulose overlay media (R&D Systems) and staining with 0.5 mg/ml thiazolyl blue tetrazolium bromide (MTT; Sigma Aldrich) solution in PBS for 3 hours at 37°C. Paraffin-embedded lung samples were sectioned at 5 μm, and stained with polyclonal anti-RSV antibody that recognizes RSV antigen from RSV A and B isolates (Abcam) and antibodies using an immunoperoxidase technique (Vectastain ABC Elite kit, Vector Laboratories, Burlingame, CA) to determine areas of lung infected.
Foronjy R.F., Ochieng P.O., Salathe M.A., Dabo A.J., Eden E., Baumlin N., Cummins N., Barik S., Campos M., Thorp E.B, & Geraghty P. (2016). Protein tyrosine phosphatase 1B negatively regulates S100A9-mediated lung damage during respiratory syncytial virus exacerbations. Mucosal immunology, 9(5), 1317-1329.
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2

Quantifying Viral Titers and Lung Infection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Infectious virus titers were determined by plaque assays. Lungs of infected mice were excised and homogenized using a mechanical homogenizer (Kinematica, Bohemia, NY, USA). The viral titers in the homogenates were quantified by plaque assay on epithelial cells using methyl cellulose overlay media (R&D Systems) and staining with 0.5 mg/ml thiazolyl blue tetrazolium bromide (MTT; Sigma Aldrich) solution in PBS for 3 hours at 37°C. Paraffin-embedded lung samples were sectioned at 5 μm, and stained with polyclonal anti-RSV antibody that recognizes RSV antigen from RSV A and B isolates (Abcam) and antibodies using an immunoperoxidase technique (Vectastain ABC Elite kit, Vector Laboratories, Burlingame, CA) to determine areas of lung infected.
Foronjy R.F., Ochieng P.O., Salathe M.A., Dabo A.J., Eden E., Baumlin N., Cummins N., Barik S., Campos M., Thorp E.B, & Geraghty P. (2016). Protein tyrosine phosphatase 1B negatively regulates S100A9-mediated lung damage during respiratory syncytial virus exacerbations. Mucosal immunology, 9(5), 1317-1329.
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