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Calponin clone calp

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Sourced in Denmark, Azerbaijan, United States

Calponin (clone CALP) is a protein that functions as a regulatory element in smooth muscle contraction. It is a useful marker for the identification and characterization of smooth muscle cells in various tissues and research applications.

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Lab products found in correlation

S100 clone 4c4.9, by Roche (2 mentions) P63 clone 4a4, by Agilent Technologies (1 mentions) Muscle specific actin clone hhf35, by Roche (1 mentions)

3 protocols using calponin clone calp

1

Immunohistochemical Staining Protocol

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For immunohistochemical analyses, 4 μm-thick sections were obtained and deparaffinized in xylene and then hydrated in a graded series of alcohol. All cases with available tissue were stained using the corresponding antibodies. Primary antibodies included DOG1 (clone SP31, Spring Bioscience, USA; dilution 1 : 100) [17 (link)], calponin (clone CALP, DAKO, Denmark; dilution 1 : 300), SMMHC (M3558, DAKO, Denmark; dilution 1 : 200), and P63 (clone 4A4, DAKO, Denmark; dilution 1 : 100). Staining was then performed using the Envision system for 30 min at room temperature. Finally, samples were incubated with diaminobenzidine peroxidase substrate to give a brown stain, counter-stained with hematoxylin, and mounted with coverslips.
Cheng H., Yang S., Qu Z., Zhou S, & Ruan Q. (2016). Novel Use for DOG1 in Discriminating Breast Invasive Carcinoma from Noninvasive Breast Lesions. Disease Markers, 2016, 5628176.
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2

Immunohistochemical Profiling of Tissue Samples

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Immunohistochemical studies were performed on five-micrometer thick sections prepared from formalin-fixed and paraffin embedded tissue. Most of the immunohistochemistry was done at The Johns Hopkins Hospital, where the primary antibodies were: p16 (clone INK4a; MTM Laboratories, Heidelberg, Germany); p63 (clone 4A4; Cell Marque Corp., Rocklin, CA); p40 (clone Ab-1; Oncogene Research Products, Cambridge, MA), muscle specific actin (clone HHF35; Ventana; Phoenix, AZ); calponin (clone CALP; Dako, Carpinteria, CA); S100 (clone 4C4.9; Ventana); c-kit (clone YR145; Cell Marque); and AE1/AE3 (clone PCK26; Ventana). For a minority of cases, immunohistochemistry had been done at an outside institution and the reported results were recorded. As a surrogate marker for the presence of HPV, p16 immunostaining was regarded as positive only if staining was both nuclear and cytoplasmic, and present in ≥70% of the tumor cells.
Bishop J.A., Andreasen S., Hang J.F., Bullock M.J., Chen T.Y., Franchi A., Garcia J.J., Gnepp D.R., Gomez-Fernandez C.R., Ihrler S., Kuo Y.J., Lewis JS J.r., Magliocca K.R., Pambuccian S., Sandison A., Uro-Coste E., Stelow E., Kiss K, & Westra W.H. (2017). HPV-related Multiphenotypic Sinonasal Carcinoma: An Expanded Series of 49 Cases of the Tumor Formerly Known as HPV-related Carcinoma with Adenoid Cystic Carcinoma-Like Features. The American journal of surgical pathology, 41(12), 1690-1701.
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3

Immunohistochemistry of Tumor Markers

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Immunohistochemistry was performed by using anti VEGF, COX-2, EGFR, ProEx-C and TERT antibodies [21] [22] [23] [24] [25] . Vascular endothelial growth factor in a 1/20 dilution (a mouse monoclonal antibody (H11) Catalogue # MA5-13182, Invitrogen, Thermo Fisher Scientific, USA), COX-2 a rabbit monoclonal anti-COX-2 antibody (diluted 1: 50; BIOCARE MEDICAL, USA), EGFR in 2 µg/ml dilution (a mouse monoclonal antibody (JH121) Catalogue # MA5-13070, Invitrogen, Thermo Fisher Scientific, USA) ProExC (prediluted, clone MCM2 26H6.19, MCM2 27C5.6, TOP2A SWT3D1; TriPath Imaging Inc, Burlington, NC), TERT, anti-telomerase catalytic subunit (RABBIT) antibody-600-401-252S (Rockland Immunochemicals, Inc., Limerick, PA, USA), muscle-specific actin (clone HHF35; Ventana), calponin (clone CALP; Dako), and S100 (clone 4C4.9; Ventana [26] [27] [28] [29] [30] .
Hanafy S., Elhasadi I., Alabiad M., Refaat M., Bakry A., Abdulmageed A., Shalaby A., Jaber F.A, & Gobran M.A. (2022). High-risk human papillomavirus enhances the expression of COX-2 VEGF, EGFR, ProEx-C, and TERT proteins in human papillomavirus-related multiphenotypic sinonasal carcinoma through activation of PI3K/Akt, pRb, and TERT signalling pathways. Polish journal of pathology : official journal of the Polish Society of Pathologists, 73(4).
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