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Legend max human il 17a elisa kit

Manufactured by BioLegend
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Sourced in Sweden, United States

The LEGEND MAX™ Human IL-17A ELISA Kit is a quantitative sandwich immunoassay designed for the measurement of human interleukin-17A (IL-17A) levels in cell culture supernatants, serum, and plasma samples.

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Lab products found in correlation

Elisapro kit, by Mabtech (1 mentions) Spectramax i3x detection system, by Molecular Devices (1 mentions) Envision 2104 multilabel plate reader, by PerkinElmer (1 mentions) Legend max mouse il 17a elisa kit, by BioLegend (1 mentions) Legendplex panels, by BioLegend (1 mentions) Facsaria 3, by BD (1 mentions)

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IL-17A ELISA kit Human IL-17A ELISA MAX kits IL-17A ELISAs

5 protocols using legend max human il 17a elisa kit

1

Serum IL-10 and IL-17 ELISA Assays

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IL-10 and IL-17 levels were determined in serial serum samples, which were collected daily from the time of admission to discharge, using the human IL-10 enzyme linked immunosorbent assay (ELISA) PRO kit (Mabtech, Sweden) and LEGEND MAX™ Human IL-17A ELISA Kit (BioLegend, USA). The ELISAs were performed and results were interpreted according to the manufacturer’s instructions.
Fernando S., Wijewickrama A., Gomes L., Punchihewa C.T., Madusanka S.D., Dissanayake H., Jeewandara C., Peiris H., Ogg G.S, & Malavige G.N. (2016). Patterns and causes of liver involvement in acute dengue infection. BMC Infectious Diseases, 16, 319.
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2

Quantification of Serum IL-17A

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For cytokine analysis, serum was separated from the blood of each patient, and culture supernatants were collected at the indicated times after cell stimulation. The level of IL-17A in the serum and supernatant samples were detected using the LEGEND MAX™ Human IL-17A ELISA Kit (BioLegend, San Diego, USA) according to the manufacturer's protocols. Absorbance was measured at 450 nm using the SpectraMax i3x detection system (Molecular Devices, California, USA).
Bao J., Cui D., Wang X., Zou Q., Zhao D., Zheng S., Yu F., Huang L., Dong Y., Yang X., Xie G., Chen W, & Chen Y. (2019). Decreased Frequencies of Th17 and Tc17 Cells in Patients Infected with Avian Influenza A (H7N9) Virus. Journal of Immunology Research, 2019, 1418251.
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3

Quantification of Serum Cytokine Levels

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Serum concentrations of IL‐22, IL‐22BP, and IL‐17A were quantified using the Human IL‐22 Quantikine ELISA (enzyme‐linked immunosorbent assay) Kit (R & D Systems, Minneapolis, MN), the Human IL‐22BP/IL‐22RA2 ELISA Kit (Lifespan Biosciences, Seattle, WA), and the LEGEND MAX Human IL‐17A ELISA Kit (BioLegend, San Diego, CA), according to manufacturers’ instructions. Samples and standard curve values were measured at 450 nm on an EnVision 2104 Multilabel Plate Reader (PerkinElmer, Waltham, MA). The specificity of the IL‐22 and IL‐22BP ELISA with respect to detection of IL‐22BP and IL‐22 (and vice versa) was assessed (Supporting Table S4). The presence of very high concentrations of IL‐22BP interfered with the IL‐22 ELISA (in line with the data sheets of all available IL‐22 ELISAs) with the consequence that IL‐22 serum concentrations might be slightly underestimated.
Schwarzkopf K., Rüschenbaum S., Barat S., Cai C., Mücke M.M., Fitting D., Weigert A., Brüne B., Zeuzem S., Welsch C, & Lange C.M. (2019). IL‐22 and IL‐22‐Binding Protein Are Associated With Development of and Mortality From Acute‐on‐Chronic Liver Failure. Hepatology Communications, 3(3), 392-405.
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4

Quantification of IL-17A in Plasma and Serum

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Secreted levels of human or mouse IL-17A in plasma or serum were determined according to the manufacturer’s instructions (LEGEND MAX Human IL-17A ELISA Kit, LEGEND MAX Mouse IL-17A ELISA Kit, BioLegend). For human samples, plasma samples from patients with psoriasis were used as internal reference controls. For multiplex quantification of cytokines, the bead-based LEGENDplex panels (Human Th17 7-plex Panel; Human Th 12-plex Panel, Mouse Th17 7-plex Panel; IL-1β, IL-23 and IL-12p70 from the Inflammation Panel 1; granzyme A and granzyme B from the CD8/NK Panel, predefined and custom-designed mix-and-match system from BioLegend) were used according to the manufacturer’s instructions. Flow cytometry reading was performed on the FACSAria III (BD). Mean fluorescence intensity values were recorded using LEGENDplex analysis software (version 2021.07.01), and cytokine concentrations (pg ml−1) were interpolated from a five-parameter logistic non-linear curve model using a separate standard curve for each cytokine. For prognostic stratification of IL-17A plasma levels, an optimal cut point was determined in each dataset separately using X-tile28 (link).
Váraljai R., Zimmer L., Al-Matary Y., Kaptein P., Albrecht L.J., Shannan B., Brase J.C., Gusenleitner D., Amaral T., Wyss N., Utikal J., Flatz L., Rambow F., Reinhardt H.C., Dick J., Engel D.R., Horn S., Ugurel S., Sondermann W., Livingstone E., Sucker A., Paschen A., Zhao F., Placke J.M., Klose J.M., Fendler W.P., Thommen D.S., Helfrich I., Schadendorf D, & Roesch A. (2023). Interleukin 17 signaling supports clinical benefit of dual CTLA-4 and PD-1 checkpoint inhibition in melanoma. Nature Cancer, 4(9), 1292-1308.
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5

Plasma IL-17A and hs-CRP Quantification

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Blood samples collected in tubes containing EDTA were centrifuged at 3000g for 15 min at 4°C to isolate the plasma. Plasma samples were stored at −80°C till further use. Plasma levels of IL-17A were measured in duplicate using Legend Max™ Human IL-17A ELISA kit (No. 433917, BioLegend, San Diego, CA). The detection limit was 0.8 pg/mL and the lowest concentration for standard sample was 3.9 pg/mL. The mean intraassay and the mean interassay coefficients of variation were 5.4% and 7.9%, respectively. High sensitivity C reactive protein (hs-CRP) levels were obtained from medical records.
Sandip C., Tan L., Huang J., Li Q., Ni L., Cianflone K, & Wang D.W. (2016). Common variants in IL-17A/IL-17RA axis contribute to predisposition to and progression of congestive heart failure. Medicine, 95(27), e4105.
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