Sb 705498

To activate TRPV1, we administered the TRPV1 activator capsaicin (HY-10488; MedChem Express, Shanghai, China) intraperitoneally at a concentration of 10 mg/kg 1 hour prior to KHES on Day 7.³²
Conversely, to inhibit TRPV1, we administered the inhibitor SB-705498 (HY10633; MedChem Express, Shanghai, China) orally at a concentration of 10 mg/kg on Day 7.^{33 (link)}
In both cases, we used 20% dimethyl sulfoxide as the vehicle.

All salts were supplied by Sangon Biotech. Glucose, caffeine, carbachol, and indomethacin were purchased from Sigma-Aldrich. Capsaicin, ouabain, SB705498, RN1734, RN1747, U73343, HC067047, GSK1016790A, methyl arachidonyl fluorophosphonate, AA, miconazole, and nystatin were purchased from MedChemExpress (MCE; Monmouth Junction). DSS was from MP Biomedicals (MP Bio). U73122 was from Tocris Bioscience (Tocris). Amiloride hydrochloride was from Molecular Probes, Inc (Molecular Probes). TNF-α and Fura-2/AM (Cat# F1221, Invitrogen) was from Invitrogen. TRPV4 (Abcam Cat# ab39260, RRID: AB_1143677), alpha 1 Sodium Potassium ATPase (phospho Y10) (Abcam Cat# ab124677, RRID: AB_10974461), Alexa Fluor 488 labeled anti-rabbit (Abcam Cat# ab150077, RRID: AB_2630356) antibodies, and MPO Assay Kit were from Abcam. Anti-GAPDH (Proteintech Cat# 60004-1-Ig, RRID: AB_2107436) was from Proteintech.