Sample cDNA concentration was measured using the Quant-iT PicoGreen dsDNA assay kit and black Nunc F96 MicroWell polystyrene plates (ThermoFisher Scientific) according to the manufacturer’s instructions, except that we used half of the recommended reaction volumes to reduce costs. Fluorescence measurements were recorded on a Hidex Sense instrument (Hidex). To assess cDNA size distribution, eleven samples were randomly selected from each plate, loaded on a HS DNA chip and run on a 2100 Bioanalyzer System (software vB.02.10.51764, Agilent).
Black nunc f96 microwell polystyrene plates
Manufactured by Thermo Fisher Scientific
The Black Nunc™ F96 MicroWell™ Polystyrene Plates are a type of microplate made of polystyrene material. The plates have 96 wells and are black in color.
Automatically generated - may contain errors
3 protocols using black nunc f96 microwell polystyrene plates
1
Quantifying cDNA Concentration and Size
2
cDNA Size Distribution Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Sample concentrations were quantified by using the Quant-iT™ PicoGreen™ dsDNA Assay Kit (ThermoFisher Scientific) and performed in black Nunc™ F96 MicroWell™ Polystyrene Plates (ThermoFisher Scientific), according to the manufacturer's instructions, except that all reaction volumes were cut by half to reduce costs. Fluorescence measurements were recorded on a Hidex Sense instrument (Hidex). In order to assess cDNA size distribution, 11 samples from each plate were randomly chosen, loaded on a High Sensitivity DNA chip and run on a 2100
Bioanalyzer System (Agilent Technologies). As a rule of thumb, the criteria for deciding whether an experiment was worth to be followed up were the following: average size distribution around 1.5-2.0 Kb, low proportion of short fragments (<400 bp) and absence of residual primers/primer dimers (<200 bp).
Bioanalyzer System (Agilent Technologies). As a rule of thumb, the criteria for deciding whether an experiment was worth to be followed up were the following: average size distribution around 1.5-2.0 Kb, low proportion of short fragments (<400 bp) and absence of residual primers/primer dimers (<200 bp).
3
Quantifying cDNA Size Distribution
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sample concentrations were quantified by using the Quant-iT™ PicoGreen™ dsDNA Assay Kit (ThermoFisher Scientific) and performed in black Nunc™ F96 MicroWell™ Polystyrene Plates (ThermoFisher Scientific), according to the manufacturer's instructions, except that all reaction volumes were cut by half to reduce costs. Fluorescence measurements were recorded on a Hidex Sense instrument (Hidex). In order to assess cDNA size distribution, 11 samples from each plate were randomly chosen, loaded on a High Sensitivity DNA chip and run on a 2100 Bioanalyzer System (Agilent Technologies). As a rule of thumb, the criteria for deciding whether an experiment was worth to be followed up were the following: average size distribution around 1.5-2.0 Kb, low proportion of short fragments (<400 bp) and absence of residual primers/primer dimers (<200 bp).
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