Hplc grade methanol

Manufactured by BD

Sourced in United Kingdom

HPLC grade methanol is a high-purity solvent commonly used in high-performance liquid chromatography (HPLC) applications. It meets the requirements for HPLC analysis, providing consistent and reliable performance.

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Lab products found in correlation

2 protocols using hplc grade methanol

Tacrolimus Extraction and PLGA Formulation

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Tacrolimus was extracted from expired Prograf 5 mg capsules (Batch # 7241), as previously described^{55 (link)}. Ploy (D, L-lactide-co-glycolide; lactide: glycolide (50:50) (PLGA)) with Mw 30,000–60,000 and Poloxamer-188 were purchased from Sigma Aldrich Co. (St. Louis MO, USA). Chloroform and dichloromethane (DCM) were purchased from MERK (Darmstadt, F.R. Germany) and PANREAC QUIMICA SA, (Barcelona, Spain), respectively. Polyvinyl alcohol (PVA, Mw 17,200) and acetone were purchased from AVON- CHEM Ltd. (Wellington House, Waterloo St. West, Macclesfield, Cheshire, UK). The HPLC grade methanol was obtained from BDH Ltd. (Poole, England). Phosphate buffer saline was obtained from GIBCO, Life Technologies Ltd. (Paisley, Scotland). Acetonitrile (HiPerSolv Chromanorm, HPLC-grade) were purchased from BDH, PROLABO1, LEUVEN, EC. Milli-Q water purifier (Millipore, France) was used for purified water and all other used chemicals were analytical grade and solvents were HPLC grade. Diagnostic kits for creatinine, blood urea, uric acid and potassium (Giesse Diagnostics, Rome, Italy).

Alshamsan A., Binkhathlan Z., Kalam M.A., Qamar W., Kfouri H., Alghonaim M, & Lavasanifar A. (2020). Mitigation of Tacrolimus-Associated Nephrotoxicity by PLGA Nanoparticulate Delivery Following Multiple Dosing to Mice while Maintaining its Immunosuppressive Activity. Scientific Reports, 10, 6675.

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Neurochemical Analysis of Caffeine and Nicotine

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Caffeine was obtained from Global Chemie (Mumbai, India). It was dissolved in saline. Nicotine is nicotine hydrogen tartrate salt and was obtained from BDH Chemicals Ltd (England) and dissolved in saline. Thiobarbituric acid, trichloroacetic acid, perchloric acid, triethylamine, absolute ethyl alcohol, sulfanilamide, and N-1-naphthyl ethylene diamine were supplied by Sigma Aldrich. In addition, glutathione, acetylthiocho-line iodide, ethylene diamine tetraacetic acid, 5,50-dithiobis-(2-nitrobenzoic acid) (DTNB), and phosphate buffers were obtained from Sigma Aldrich. Analytical-grade glacial acetic acid, dansyl chloride, lithium carbonate, and high-performance liquid chromatography (HPLC)-grade acetonitrile were purchased from Fisher (UK). Standard amino acids and HPLC-grade methanol were supplied by BDH (England).

Mourad I.M., Noor N.A., Mohammed H.S., Aboul Ezz H.S, & Khadrawy Y.A. (2021). A Neurochemical and Electrophysiological Study on the Combined Effects of Caffeine and Nicotine in the Cortex of Rats. Basic and Clinical Neuroscience, 12(5), 681-692.

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