Legs and details of the male and female genitalia were drawn by means of an Abbe’s drawing apparatus on a compound microscope (Zeiss Jenaval) at magnification 130–500×. Wings were photographed on an Olympus BX51 compound microscope with an attached digital camera (Canon EOS 1200D). Whole adult (dry-mounted) specimens and heads were photographed by means of a Canon EOS 5D Mark III digital camera with a Nikon CFI Plan 10×/0.25NA 10.5mm WD objective attached to a Canon EF 70–200mm f/4L USM zoom lens. The specimen photographed by means of the latter equipment was repositioned upwards between each exposure using a Cognisys StackShot Macro Rail and the final photograph was compiled from multiple layers (~ 40) using Helicon Focus Pro 7.0.2. The final images were edited in Adobe Photoshop CS6. All morphological illustrations were prepared by the first author.
Bx51 compound microscope
Manufactured by Canon
The BX51 is a compound microscope manufactured by Canon. It is designed for scientific and laboratory applications, providing high-quality magnification and imaging capabilities. The BX51 features advanced optics, including infinity-corrected objectives, to deliver clear, detailed images. Its core function is to enable precise observation and analysis of samples under high magnification.
Automatically generated - may contain errors
Lab products found in correlation
Cfi plan 10 0.25na 10 5mm wd objective, by Canon (2 mentions)
Compound microscope, by Zeiss (1 mentions)
Eos 1200d, by Canon (1 mentions)
Ef 70 200mm f 4l usm zoom lens, by Canon (1 mentions)
Stackshot macro rail, by Helicon (1 mentions)
Focus pro 7, by Helicon (1 mentions)
7d camera, by Canon (1 mentions)
Szx16 stereomicroscope, by Olympus (1 mentions)
5d mark 2, by Canon (1 mentions)
Bx53 compound microscope, by Olympus (1 mentions)
Sz51 stereomicroscope, by Olympus (1 mentions)
4 protocols using bx51 compound microscope
1
Detailed Morphological Documentation of Insects
2
Stereomicroscopic Examination of Spider Genitalia
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Specimens were studied using an Olympus SZX16 stereomicroscope. Anatomical details were examined and photographed with on Olympus BX51 compound microscope and a Canon 7D camera. Genitalia were cleared in boiling KOH for a few minutes to dissolve soft tissues. All the specimens were deposited at the
Abbreviations used:
ALE
AME
PLE
PME
MOA
PMS
PLS
Centre for Behavioural Ecology and Evolution
Abbreviations used:
anterior lateral eye
anterior median eye
posterior lateral eye
posterior median eye
median ocular area
posterior median spinneret
posterior lateral spinneret
3
Detailed Morphological Documentation Protocol
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Legs were drawn on squared paper using a Reichert binocular microscope with an ocular screen. Details of the male and female genitalia and all preimaginal stages were drawn by means of an Abbe's drawing apparatus on a compound microscope (JENAVAL) at larger magnification (130-500 ×). Wings were photographed using an Olympus BX51 compound microscope with an attached digital camera (Canon EOS 1200D). Whole adult specimens and their details were photographed by P. Krásenský by means of a Canon 5D Mark II digital camera with a Nikon CFI Plan 10 × / 0.25NA 10.5 mm WD objective attached on a Canon EF 70-200 mm f / 4L USM lens. The specimens were moved upwards between each exposure using a WeMacro Rail (http://www.wemacro.com/, each step was 0.01 mm) and the final photographs were stacked from multiple layers using Zerene Stacker. The final images were edited in Adobe Photoshop CS6.
4
Arachnid Specimen Collection and Examination
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Specimens were collected mainly by beating shrubs and screening leaf-litter. All specimens were preserved in 75% ethanol and are deposited in the museum of Tongren University (TRU), Tongren, China except the types of Synagelides xingdouensis sp. nov. and the new material of S. forkiforma Yang, Zhu & Song, 2007, which are deposited at the College of Life Sciences, Hunan Normal University (HNU), Changsha, Hunan, China.
The specimens were examined with an Olympus SZ51 stereo microscope. After dissecting from the body, epigynes were cleared in trypsin enzyme solution before examination and imaging. Left male palps, legs I and chelicerae were used for illustration. Photos were taken with a Kuy Nice CCD mounted on an Olympus BX51 compound microscope except the photos of Fig. 17, which were taken with a Canon G12 camera mounted on an Olympus BX53 compound microscope. Compound focus images were generated using Helicon Focus 6.7.1 and 3.1 software.
All measurements are given in millimeters. Leg measurements are giving as: total length (femur, patella + tibia, metatarsus, tarsus). References to figures in the literature are listed in lowercase type (fig.
The specimens were examined with an Olympus SZ51 stereo microscope. After dissecting from the body, epigynes were cleared in trypsin enzyme solution before examination and imaging. Left male palps, legs I and chelicerae were used for illustration. Photos were taken with a Kuy Nice CCD mounted on an Olympus BX51 compound microscope except the photos of Fig. 17, which were taken with a Canon G12 camera mounted on an Olympus BX53 compound microscope. Compound focus images were generated using Helicon Focus 6.7.1 and 3.1 software.
All measurements are given in millimeters. Leg measurements are giving as: total length (femur, patella + tibia, metatarsus, tarsus). References to figures in the literature are listed in lowercase type (fig.
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