Alexa fluor 594 coupled goat anti mouse igg antibody

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Alexa Fluor 594-coupled goat anti-mouse IgG antibody is a fluorescently-labeled secondary antibody used for detection and visualization in immunoassays and other applications. It is designed to specifically bind to mouse immunoglobulin G (IgG) antibodies, allowing for the detection and localization of target proteins or molecules in a sample.

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Lab products found in correlation

Alexa fluor 488 coupled goat anti rabbit igg antibody, by Thermo Fisher Scientific (1 mentions) Primary mouse cd68 antibody, by Santa Cruz Biotechnology (1 mentions) Anti pgp9.5 antibody, by Merck Group (1 mentions) Fv10i confocal system, by Olympus (1 mentions) Cm1510, by Leica (1 mentions)

Spelling variants of this product

Alexa Fluor 594 goat anti-mouse IgG (244 citations) Alexa Fluor 594 goat anti-mouse (177 citations) Alexa Fluor 594 anti-mouse (84 citations) Anti-mouse IgG-Alexa Fluor®594 (62 citations) Goat anti-mouse Alexa 594 (49 citations) Alexa Fluor 594 goat anti-mouse antibody (37 citations) Alexa Fluor 594 Goat Anti-Mouse IgG Antibody (11 citations) Alexa Fluor goat anti-mouse IgG (10 citations) Alexa Fluor 594 anti-goat IgG (7 citations) 594 Goat Anti Mouse (2 citations)

2 protocols using alexa fluor 594 coupled goat anti mouse igg antibody

Immunofluorescent Detection of Monocytes and Macrophages

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For the detection of monocytes/macrophages, the sections were incubated with the primary mouse CD68 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA). After washing, Alexa Fluor 594‐coupled goat anti‐mouse immunoglobulin G (IgG) antibody (Invitrogen, Carlsbad, CA, USA) and 4′,6‐diamidino‐2‐phenylindole were applied.
To characterize macrophages, the sections were double‐stained with the primary mouse CD68 antibody and rabbit CD206 antibody (Santa Cruz). After washing, Alexa Fluor 594‐coupled goat anti‐mouse IgG antibody, Alexa Fluor 488‐coupled goat anti‐rabbit IgG antibody (Invitrogen) and 4′,6‐diamidino‐2‐phenylindole were applied. Slides were analyzed under a fluorescence microscope.

Omi M., Hata M., Nakamura N., Miyabe M., Kobayashi Y., Kamiya H., Nakamura J., Ozawa S., Tanaka Y., Takebe J., Matsubara T, & Naruse K. (2015). Transplantation of dental pulp stem cells suppressed inflammation in sciatic nerves by promoting macrophage polarization towards anti‐inflammation phenotypes and ameliorated diabetic polyneuropathy. Journal of Diabetes Investigation, 7(4), 485-496.

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Evaluating Intraepidermal Nerve Fiber Density

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After the fixation of the footpads, tissues were immersed in an OCT compound (Sakura Finetechnical, Tokyo, Japan) containing liquid nitrogen and isopentane. Three longitudinal 25-μm thick footpad sections from each rat were cut on a cryostat (Leica CM 1510 S). The sections were incubated overnight at 4 °C with the primary antibody (anti-PGP9.5 antibody; Millipore, Tokyo, Japan). Alexa Fluor 594-coupled goat anti-mouse IgG antibody (Invitrogen, Carlsbad, CA, USA) was applied as the second antibody. IENFD was assessed as previously reported [13 (link)]. Nerve fibers were counted blindly by three independent investigators under an FV10i confocal system (Olympus, Tokyo, Japan) and the average values were used.

Omi M., Hata M., Nakamura N., Miyabe M., Ozawa S., Nukada H., Tsukamoto M., Sango K., Himeno T., Kamiya H., Nakamura J., Takebe J., Matsubara T, & Naruse K. (2017). Transplantation of dental pulp stem cells improves long-term diabetic polyneuropathy together with improvement of nerve morphometrical evaluation. Stem Cell Research & Therapy, 8, 279.

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