Cd8 percp cy5.5 clone 53 6 7

Manufactured by BioLegend

CD8-PerCP-Cy5.5 (clone 53-6.7) is a fluorescently-conjugated monoclonal antibody that binds to the CD8 cell surface antigen. It is designed for use in flow cytometry applications.

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Lab products found in correlation

Strataclean resin, by Agilent Technologies (1 mentions) Atp tlrl atpl, by InvivoGen (1 mentions) Aicar s1802, by Selleck Chemicals (1 mentions) Compound c p5499, by Merck Group (1 mentions) Anti ha h6908, by Merck Group (1 mentions) Pt172 ampk, by Cell Signaling Technology (1 mentions) Anti gsdmd ab219800, by Abcam (1 mentions) Anti il18 a1115, by ABclonal (1 mentions) Cd4 apc clone rm4 5, by BioLegend (1 mentions) Facsverse instrument, by BD (1 mentions) Dynabeads untouched mouse cd8 t cell kit, by Thermo Fisher Scientific (1 mentions) Cd127 pecy7 clone a019d5, by BioLegend (1 mentions) Cd25 apc cy7 clone bc96, by BioLegend (1 mentions) Cd8 v450 clone rpa t8, by BD (1 mentions) Cd45ro apc clone uchl1, by BioLegend (1 mentions) Zombie live dead stain, by BioLegend (1 mentions) Cd8 percp cy5.5 clone sk1, by BioLegend (1 mentions)

Spelling variants of this product

PerCP-Cy5.5 (133 citations) CD8-PerCP-Cy5.5 (54 citations) CD8 (clone 53-6.7, (39 citations) CD8 (53-6.7, (38 citations) CD8-PerCP (30 citations) Clone 53-6.7 (21 citations) PerCP/Cy5.5-CD8 (7 citations) Anti-CD8-PerCP/Cy5.5 (clone 53–6.7) (3 citations) CD8-PerCP (clone-53-6.7, (2 citations) CD8 (53-5.8, (2 citations)

2 protocols using cd8 percp cy5.5 clone 53 6 7

AMPK Signaling Pathway Modulation

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LPS O111:B4 (L2630), Doxycycline (D3447), 2-Deoxy-D-glucose (D8375) and compound C (P5499) were obtained from Sigma-Aldrich. Subcellular protein fractionation kit (78840) was obtained from Thermo Fisher. Metformin (S1950) and AICAR (S1802) were obtained from Selleck Chemicals. ATP (tlrl-atpl) was obtained from InvivoGen. StrataClean resin (400714) and chemical competent cells (200315) were obtained from Agilent.
For immunoblot, AMPKα1(#2795), AMPKα2 (#2757), AMPKα1/2 (#5831) and pT172-AMPK (#2535) were purchased from Cell Signaling Technology. Anti-Flag (F1804), anti-Flag (F7425) and anti-HA (H6908) were obtained from Sigma-Aldrich. Anti-GSDMD (ab219800) and anti-Caspase-1 (ab108362) were obtained from Abcam. Anti-β-Actin (sc-47778), anti-GST (sc-138) and anti-Na/K-ATPase α1 (sc-21712) were purchased from Santa Cruz Biotechnology. Anti-IL18 (A1115) was obtained from Abclonal Technology. phospho-GSDMD S46 was generated by Abclonal Technology.
For FCAS analysis, CD45-APC-Cy7(clone 30-F11), CD3-FITC (clone 145-2C11), CD8-PerCP-Cy5.5 (clone 53-6.7) and CD4-APC (clone RM4-5) were obtained from BioLegend.

Chu X., Xiao X., Wang G., Uosef A., Lou X., Arnold P., Wang Y., Kong G., Wen M., Minze L.J, & Li X.C. (2023). Gasdermin D-mediated pyroptosis is regulated by AMPK-mediated phosphorylation in tumor cells. Cell Death & Disease, 14(7), 469.

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Comprehensive Immunophenotyping by Flow Cytometry

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Flow cytometry was performed on a BD FACSverse instrument. Antibodies used for mouse cell analysis: CD127-PE/V450 clone A7R34, CD25-FITC clone 7D4, CD44-PerCPCy5.5/FITC clone IM7, CD69-PECy7 clone HI.2F3, CD8-PerCPCy5.5 clone 53-6.7, CD8-PECy7 clone YTS156.7.7, PD1-PerCPCy5.5 clone 29F.1A12 Vβ13-PE/APC clone MR12-3/MR12-4 (Biolegend), CD4-APCCy7 clone RM4-5, CD62L-APC clone MEL-14, and KLRG1-APC/V450 clone 2F1 (BD). Antibodies used for human cell analysis: CD127-PECy7 clone A019D5, CD25-APCCy7 clone BC96, CD4-APCCy7 OKT4, CD45RO-APC clone UCHL1, CD62L-FITC clone DREG-56, CD8-PerCPCy5.5 clone SK1, TIM3-PE clone F38-2E2 (biolegend), CCR7-PECy7 clone CCR7, CD8-V450 clone RPA-T8, and PD1-FITC clone M1H4 (BD Biosciences). Cell viability assessed via Zombie Live/Dead Stain (biolegend). Data analyzed using Flowjo 10 software (Tree Star). For experiments with sorted cells, CD8⁺Vb13⁺ T cells were sorted from pmel-1 cultures using Dynabeads untouched mouse CD8⁺ T cell kit (Invitrogen) and a FACSAria cell sorting machine (BD Biosciences).

Bowers J.S., Majchrzak K., Nelson M.H., Aksoy B.A., Wyatt M.M., Smith A.S., Bailey S.R., Neal L.R., Hammerbacher J.E, & Paulos C.M. (2017). PI3Kδ Inhibition Enhances the Antitumor Fitness of Adoptively Transferred CD8+ T Cells. Frontiers in Immunology, 8, 1221.

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