An inverted microscope (Axio Observer.Z1 from Zeiss, 2.3.64.0) with 20x air objective (NA 0.8) was used for image acquisition. FITC, mCherry, calcein AM, ethidium homodimer and 5-CNF filters were used to inspect the imprint of the PLL-FITC coated stamps as well as the viability of the immobilised cells. Initial image processing was performed using the Zeiss image analysis software (2.3.64.0).
Image analysis software 2.3.64.0
Manufactured by Zeiss
Zeiss image analysis software (2.3.64.0) is a software application designed for the analysis and processing of digital images. The software provides tools for image segmentation, object detection, and measurement. It is compatible with a variety of image file formats and can be used in conjunction with Zeiss imaging hardware.
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Lab products found in correlation
4 protocols using image analysis software 2.3.64.0
1
Microscopic Imaging of PLL-FITC Coated Cells
2
Fluorescence Microscopy of Plasmid-Expressing Cells
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Cell cultures of E. coli and S. cerevisiae carrying the respective plasmids expressing mCherry and the V1 isoform were placed between glass slides for the microscopic analysis. An inverted fluorescence microscope (Zeiss Axio Observer. Z1, 2.3.64.0) with a ×20 air objective (NA 0.8) was used to detect mCherry and was localized within the cells using the bright field filter. Image processing was performed using the Zeiss image analysis software (2.3.64.0).
3
Fluorescence Microscopy
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The strains carrying the weakest
single-gene GES (P1) and the weakest paired-gene GES (P2) were analyzed
by fluorescence microscopy to confirm protein expression and cellular
colocalization. One microliter of overnight culture was analyzed with
an inverted microscope (Zeiss Axio Observer.Z1, 14 2.3.64.0) possessing
a 20× air objective (NA 0.8). The GFP and mCherry filters were
applied to measure the fluorescence of both proteins. Image processing
was performed with Zeiss image analysis software (2.3.64.0).
single-gene GES (P1) and the weakest paired-gene GES (P2) were analyzed
by fluorescence microscopy to confirm protein expression and cellular
colocalization. One microliter of overnight culture was analyzed with
an inverted microscope (Zeiss Axio Observer.Z1, 14 2.3.64.0) possessing
a 20× air objective (NA 0.8). The GFP and mCherry filters were
applied to measure the fluorescence of both proteins. Image processing
was performed with Zeiss image analysis software (2.3.64.0).
4
Fluorescence Microscopy of GES Proteins
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The strains carrying the weakest single-gene GES (P1) and the weakest paired-gene GES (P2) were analyzed by fluorescence microscopy to confirm protein expression and cellular co-localization. One microliter of overnight culture was analyzed with an inverted microscope (Zeiss Axio Observer.Z1, 14 2.3.64.0) possessing a 20x air objective (NA 0.8). The GFP and mCherry filters were applied to measure the fluorescence of both proteins. Image processing was performed with Zeiss image analysis software (2.3.64.0).
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