Kanamycin kan

Manufactured by GoldBio

Kanamycin (Kan) is an aminoglycoside antibiotic used in molecular biology and microbiology research. It functions as a selective agent, inhibiting the growth of bacteria that do not contain the kanamycin resistance gene.

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Lab products found in correlation

Ampicillin amp, by Merck Group (5 mentions) Chloramphenicol cm, by Merck Group (5 mentions) L arabinose, by Merck Group (3 mentions) Polymyxin b, by Merck Group (3 mentions) Ciprofloxacin, by Merck Group (2 mentions) Anhydrotetracycline atc, by Takara Bio (2 mentions) Isopropyl β d 1 thiogalactopyranoside iptg, by GoldBio (1 mentions) L arabinose ara, by Merck Group (1 mentions) Mitomycin c mmc, by Merck Group (1 mentions) Halo tmr, by Promega (1 mentions) Isopropyl β d 1 thiogalactopyranoside (iptg), by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Kanamycin (79 citations)

5 protocols using kanamycin kan

Culturing Bacterial Strains for Research

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E. coli and V. cholerae strains were grown with aeration in Luria-Bertani (LB-Miller, BD-Difco) broth at 37 °C. V. parahaemolyticus strains were grown with aeration in LB with 3% NaCl at 30 °C. Strains used in the study are listed in S2 Table. Unless otherwise noted, antibiotics, were used at: 100 μg mL^-1 ampicillin (Amp, Sigma), 50 μg mL^-1 kanamycin (Kan, GoldBio), 50 μg mL^-1 polymyxin B (Pb), and 5 μg mL^-1 chloramphenicol (Cm, Sigma). L-arabinose (Sigma) was supplied at final concentrations of 0.02% or 0.2%, as indicated in the figure legends. Ciprofloxacin (Sigma) and DPO were supplied at a final concentrations of 500 ng mL^-1 and 10 μM, respectively.

Duddy O.P., Silpe J.E., Fei C, & Bassler B.L. (2023). Natural silencing of quorum-sensing activity protects Vibrio parahaemolyticus from lysis by an autoinducer-detecting phage. PLOS Genetics, 19(7), e1010809.

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Culturing E. coli, V. cholerae, and V. parahaemolyticus

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E. coli and V. cholerae strains were grown with aeration in Luria-Bertani (LB-Miller, BD-Difco) broth at 37° C. V. parahaemolyticus strains were grown with aeration in LB with 3% NaCl at 30° C. Strains used in the study are listed in Supplementary Table S2. Unless otherwise noted, antibiotics, were used at: 100 μg mL⁻¹ ampicillin (Amp, Sigma), 50 μg mL⁻¹ kanamycin (Kan, GoldBio), 50 μg mL⁻¹ polymyxin B (Pb), and 5 μg mL⁻¹ chloramphenicol (Cm, Sigma). L-arabinose (Sigma) was supplied at final concentrations of 0.02% or 0.2%, as indicated in the figure legends. Ciprofloxacin (Sigma) and DPO were supplied at a final concentrations of 500 ng mL⁻¹ and 10 μM, respectively.

Duddy O.P., Silpe J.E., Fei C, & Bassler B.L. (2023). Natural Silencing of Quorum-Sensing Activity Protects Vibrio parahaemolyticus from Lysis by an Autoinducer-Detecting Phage. bioRxiv.

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Bacterial Growth Conditions and Antibiotic Usage

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Unless otherwise indicated, E. coli, V. cholerae, V. parahaemolyticus, and V. vulnificus were grown with aeration in Luria-Bertani (LB-Miller, BD-Difco) broth at 37°C. Low salt LB (Lennox) broth w as used for S. typhimurium, and M. jannaschii was grown in Marine Broth 2216 (BD-Difco) at room temperature. M9 minimal medium supplemented with 200 mM NaCl (for V. parahaemolyticus and V. cholerae) was used where indicated. Strains used in this study are listed in Table S1. Unless otherwise noted, antibiotics, were used at: 50 U mL⁻¹ polymyxin B (Pb, Sigma), 100 μg mL⁻¹ ampicillin (Amp, Sigma), 100 μg mL⁻¹ kanamycin (Kan, GoldBio), 80 μg mL⁻¹ Zeocin (Zeo, Thermo), and 5 μg mL⁻¹ or 10 μg mL⁻¹ chloramphenicol (Cm, Sigma). Cm concentration was 10 μg mL⁻¹ when it was the only antibiotic present and 5 μg mL⁻¹ when used in conjunction with other antibiotics. When multiple plasmids were simultaneously present within a single strain, care was exercised to limit the number of passages. Inducers were used as follows: E. coli: 250 ng mL⁻¹ mitomycin C (MMC, Sigma), 100 ng mL⁻¹ anhydrotetracycline (aTc, Clontech), 0.2% L-arabinose (ara, Sigma), 0.5 mM Isopropyl β-D-1-thiogalactopyranoside (IPTG, GoldBio). Vibrios: 50 ng mL⁻¹ MMC, 10 ng mL⁻¹ aTc, 0.2% ara. S. typhimurium: 2 ng mL⁻¹ aTc.

Silpe J.E, & Bassler B.L. (2018). A Host-Produced Quorum-Sensing Autoinducer Controls a Phage Lysis-Lysogeny Decision. Cell, 176(1-2), 268-280.e13.

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Growth Conditions for Bacterial Strains

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All strains were grown with aeration in Luria-Bertani (LB-Miller, BD-Difco) broth at 37°C except for the E. coli lambda cI857 lysogen which was grown at 30°C. Stra ins used in this study are listed in Table S1. Unless otherwise noted, antibiotics and inducers were used at: 100 μg mL⁻¹ ampicillin (Amp, Sigma), 100 μg mL⁻¹ kanamycin (Kan, GoldBio), 5 μg mL⁻¹ chloramphenicol (Cm, Sigma), 100 ng mL⁻¹ anhydrotetracycline (aTc, Clontech), and 0.4 mM Isopropyl β-D-1-thiogalactopyranoside (IPTG, Fisher). For microscopy, HALO-TMR (Promega) and SNAP-JF₅₀₃ (Lavis Lab) were used at concentrations of 1 and 2 μM, respectively. AB solid agar consisted of 1.5% agar, 0.3 M NaCl, 50 mM MgSO₄, 0.2% casamino acids, 1 mM arginine, 1% glycerol, and 10 mM potassium phosphate at pH 7.5.

Silpe J.E., Bridges A.A., Huang X., Coronado D.R., Duddy O.P, & Bassler B.L. (2020). Separating Functions of the Phage-Encoded Quorum-Sensing-Activated Antirepressor Qtip. Cell host & microbe, 27(4), 629-641.e4.

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Bacterial Growth and Induction Conditions

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E. coli strains were grown with aeration in Luria–Bertani (LB-Miller, BD-Difco) broth. Aeromonas sp. ARM81 and V. fischeri strains were grown in LB with 3% NaCl. All strains were grown at 30 °C. Strains used in the study are listed in SI Appendix, Table S1. Unless otherwise noted, the following antibiotics and concentrations were used: 100 μg mL⁻¹ ampicillin (Amp, Sigma), 50 μg mL⁻¹ kanamycin (Kan, GoldBio), and 5 μg mL⁻¹ chloramphenicol (Cm, Sigma). Inducers were used as follows: E. coli: 200 μM isopropyl beta-D-1-thiogalactopyranoside (IPTG, GoldBio), 0.1% L-arabinose (Sigma), and 50 ng mL⁻¹ or 25 ng mL⁻¹ anhydrotetracycline (aTc, Clontech) and Aeromonas sp. ARM81: 0.1 ng mL⁻¹ aTc. HSL AIs were supplied at a final concentration of 20 μM, unless otherwise indicated.

Silpe J.E., Duddy O.P, & Bassler B.L. (2022). Natural and synthetic inhibitors of a phage-encoded quorum-sensing receptor affect phage–host dynamics in mixed bacterial communities. Proceedings of the National Academy of Sciences of the United States of America, 119(49), e2217813119.

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