Bongkrekic acid

Reagents were purchased as follows: bongkrekic acid (19079, Cayman, USA), carboxyatractyloside (19079, MCE, China), cisplatin (HY‐17394, MCE, China), calcein‐AM (17783, Sigma‐Aldrich, USA), CoCl₂ (V900021, Sigma‐Aldrich, USA), calcimycin (A23187, HY‐N6687, MCE, China), Magnesium green™ (M3733, Invitrogen, USA), STMRM, (T668, Invitrogen, USA), ATP Determination Kit (A22066, Invitrogen, USA), CCK‐8 (CK04, Dojindo, Japan), GST tag Purification Kit (P2262, Beyotime, China), MultiS Fast Mutagenesis Kit (C214, Vazym, China), 3,5‐difluoro‐L‐tyrosine (CAS 73246‐30‐7, Yiji Biotechnology, Shanghai, China).
Antibodies used included ANT1 mAb (ab110322, Abcam, UK, 1:1,000), VDAC1 mAb (Ab34726, Abcam, UK, 1:1,000), HSP60 mAb (Sc‐1052, Santa Cruz Biotechnology, USA, 1:1,000), β‐actin mAb (A5441, Sigma, Germany, 1:10,000), cyto C mAb (556433, BD, USA, 1:1,000), TIM23 mAb (11123‐1‐AP, Proteintech, USA, 1:1,000), ANT2 mAb (14671S, CST, USA, 1:2,000), VDAC2 mAb (11663‐1‐AP, Proteintech, USA, 1:1,000), caspase‐9 mAb (9502S, CST, USA, 1:1,000), Flag mAb (F1804, Sigma‐Aldrich, USA, 1:1,000), and cleaved caspase‐3 mAb (9664S, CST, USA, 1:1,000).

Unless otherwise indicated reagents were sourced from Sigma-Aldrich (St. Louis, MO, USA). Bongkrekic Acid (Cat# 19079) was obtained from Cayman (Cayman Chemical, Ann Arbor, MI, USA) and was dissolved in DMSO and used to a final 0.1% v/v ratio. Mice were obtained from Charles River Laboratories, Inc. (Wilmington, MA, USA).