Immunoprecipitation and immunoblotting were performed as described previously.24 (link) Briefly, cell lysates (50 – 100µg protein) were incubated with protein A agarose (Roche Applied Science, Mannheim, Germany) for 1h. The precleared samples were then incubated with specific antibodies at 4°C overnight. Normal rabbit IgG was used as negative control. protein A agarose beads were incubated with protease inhibitors. The immunocomplexes were washed and separated on a 4–20% SDS-PAGE gradient gel (Invitrogen, CA) and analyzed by immunoblot. The antibodies utilized were CBP/p300 (Santa Cruz, SC-32244), anti-β-catenin (BD Bioscience, Cat# 610153) and anti-γ-catenin (BD Bioscience, Cat# 610253). Antibodies for immunoblotting (50 µg total protein) were anti-BCR-ABL (clone 131), anti-CBP (clone SC-369), anti-p300 (clone SC-584), anti-survivin (clone SC-10811), all from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). Anti-α-tubulin (clone CP06) was from Calbiotech (Spring Valley, CA). UN-SCAN-IT software was used to quantify the protein bands normalized to α-tubulin.
Anti α tubulin clone cp06
Manufactured by Calbiotech
Anti-α-tubulin (clone CP06) is a monoclonal antibody that specifically binds to the alpha-tubulin subunit of microtubules. It is a useful tool for the detection and visualization of microtubules in various cell and tissue samples.
Automatically generated - may contain errors
2 protocols using anti α tubulin clone cp06
1
Immunoprecipitation and Immunoblotting of BCR-ABL Complex
2
Immunoprecipitation and Immunoblotting of BCR-ABL Complex
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immunoprecipitation and immunoblotting were performed as described previously.24 (link) Briefly, cell lysates (50 – 100µg protein) were incubated with protein A agarose (Roche Applied Science, Mannheim, Germany) for 1h. The precleared samples were then incubated with specific antibodies at 4°C overnight. Normal rabbit IgG was used as negative control. protein A agarose beads were incubated with protease inhibitors. The immunocomplexes were washed and separated on a 4–20% SDS-PAGE gradient gel (Invitrogen, CA) and analyzed by immunoblot. The antibodies utilized were CBP/p300 (Santa Cruz, SC-32244), anti-β-catenin (BD Bioscience, Cat# 610153) and anti-γ-catenin (BD Bioscience, Cat# 610253). Antibodies for immunoblotting (50 µg total protein) were anti-BCR-ABL (clone 131), anti-CBP (clone SC-369), anti-p300 (clone SC-584), anti-survivin (clone SC-10811), all from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). Anti-α-tubulin (clone CP06) was from Calbiotech (Spring Valley, CA). UN-SCAN-IT software was used to quantify the protein bands normalized to α-tubulin.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!