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Nanobret 618 ligand

Manufactured by Promega
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The NanoBRET 618 Ligand is a fluorescent compound designed for use in NanoBRET assays. It is a substrate for the NanoLuc luciferase enzyme, which can be used to measure protein-protein interactions and other biomolecular events in live cells.

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Lab products found in correlation

Prism 6, by GraphPad (1 mentions) Synergy h4, by Agilent Technologies (1 mentions) Synergy 4 instrument, by Agilent Technologies (1 mentions)

Close spelling variants of this product

HaloTag NanoBRET 618 Ligand (17 citations)

2 protocols using nanobret 618 ligand

1

NanoBRET Protein-Protein Interaction Assay

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The NanoBRET assay was performed as described by the manufacturer (Promega) and previously performed66 (link). Briefly, for protein-protein interaction assays, HL-1 transfected cells were treated with 100 nM NanoBRET 618 Ligand (Promega) and signals were detected 6 hours after treatment. Signals were detected using a Synergy H4 instrument (BioTek) and results were analysed using Prism 6.0 software (GraphPad Software, CA).
Di Mauro V., Crasto S., Colombo F.S., Di Pasquale E, & Catalucci D. (2019). Wnt signalling mediates miR-133a nuclear re-localization for the transcriptional control of Dnmt3b in cardiac cells. Scientific Reports, 9, 9320.
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2

NanoBRET Assay for Protein Interactions

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The NanoBRET Assay was performed as previously described (Rusconi et al., 2016 (link)). Briefly, transfected HEK293 cells were treated with 100 nM NanoBRET 618 Ligand (Promega), and signals were detected 6 h after treatment. Signals were detected using a Synergy 4 instrument (BioTek).
Di Mauro V., Ceriotti P., Lodola F., Salvarani N., Modica J., Bang M.L., Mazzanti A., Napolitano C., Priori S.G, & Catalucci D. (2021). Peptide-Based Targeting of the L-Type Calcium Channel Corrects the Loss-of-Function Phenotype of Two Novel Mutations of the CACNA1 Gene Associated With Brugada Syndrome. Frontiers in Physiology, 11, 616819.
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