Penicillin streptomycin solution 100

Pyropia yezoensis was obtained from Seaweed Research Center, National Institute of Fisheries Science (Mokpo, South Korea). The triglycerides (TG) assay kit and total protein (TP) assay kit were provided by Shenzhen Icubio Biomedical Technology Co., Ltd. (Shenzhen, China). TRIzol reagent was purchased from Invitrogen (Carlsbad, CA, USA). TransScript All-in-One First-Strand cDNA Synthesis Supermix for qPCR, and TransStart Top Green qPCR SuperMix were purchased from TransGen Biotech (Beijing, China). Mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose, and fucose were obtained from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). Thiazolyl blue tetrazolium bromide (MTT) and dimethyl sulfoxide were purchased from BBI Life Sciences (Shanghai, China). Minimum essential medium (MEM) and fetal bovine serum (FBS) were purchased from Gibco (Grand Island, NE, USA). Penicillin-streptomycin solution (100×) was obtained from Biosharp Life Sciences (Anhui, China). Dextran standards were purchased from China Pharmaceutical Biological Products Analysis Institute (Beijing, China). Palmitic acid (PA) was provided by Kunchuang Biotechnology (Xi’an, China). All other chemical reagents used were of analytical grade.

Drosophila melanogaster w¹¹¹⁸ was provided by Qidong Fungene Biotechnology (Jiangsu, China). Assay kits for glucose, triglyceride, and total protein were provided by Shenzhen Icubio Biomedical Technology Co., Ltd (Shenzhen, China). TRIzol reagent was purchased from Invitrogen (Carlsbad, CA, USA). PrimeScript^TM RT Master Mix (Perfect Real Time) was obtained from Takara Biomedical Technology Co., Ltd. (Dalian, China). TransStart Top Green qPCR SuperMix was purchased from TransGen Biotech (Beijing, China). Minimum essential medium (MEM), fetal bovine serum (FBS), MEM nonessential amino acids (100×), L‐glutamine (100×), and sodium pyruvate were provided by Gibco (Grand Island, NY, USA). Penicillin–streptomycin solution (100×) was obtained from Biosharp Life Sciences (Anhui, China). All other chemical reagents used in this study were of analytical grade.

The human glioma cell lines, U251, T98G, and human astroglia SVGp12 were purchased from the Procell (Wuhan, China) and were cultured in Dulbecco’s modified Eagle medium (DMEM; Servicebio) with 10% fetal bovine serum (FBS, Excel) and 10ul/ml Penicillin–Streptomycin Solution, 100× (Biosharp) at a cell incubator (Hair) at 37°C with 5% CO₂.
Takara RNAiso Plus (Takara Bio. Inc., Otsu, Shiga, Japan) was used to isolate total RNA from the tissues persevered at a temperature < −80°C according to the manual protocol. cDNA was synthesized from 1ug RNA using the HiScriptIIQ RT SuperMix for qPCR Kit (Vazyme Medical Technology). The 2^−ΔCt method was used to process qPCR data. Primer sequences used were listed as follows: GAPDH-F, 5′-GGAGCGAGATCCCTCCAAAAT-3′, GAPDH-R, 5′-GGCTGTTGTCATACTTCTCATGG-3′; C1RL-AS1-F, 5′-GGCTCCCACTGATTCTACATTAGG-3′, C1RL-AS1-R, 5′-TCCTTCTCCTTCTACTCACAGAGC-3′; LINC00968-F, 5′- GCCCAGTTGACAGGAAATGT-3′, LINC00968-R, 5′-TTGGTTCTCAATGGGATGGT-3′; miR155HG-F, 5′-GAGTGCTGAAGGCTTGCTGT-3′, miR155HG-R, 5′-TTGAACATCCCAGTGACCAG-3′; CPB2-AS1-F, 5′-GCCTAGTAGGGGGACTTCCA-3′, CPB2-AS1-R, 5′-TCCATCCTTCCCCGCTAAGA-3′; LINC00906-F, 5′- CCGAAGAACCCTCAGTAGGC-3′, LINC00906-R, 5′-GGGTGGGCTATTGCTTGCTA-3′; WDR11-AS1-F, 5′-GACCCTTTGTCTGTTGCT-3′, WDR11-AS1-R, 5′- CAGAGGCCAGTCATCCTA-3.