Orthophosphoric acid

Manufactured by BD

Sourced in United Kingdom

Orthophosphoric acid is a clear, colorless, and odorless chemical compound. It is a common inorganic acid with the chemical formula H3PO4. Orthophosphoric acid is widely used in various industries, including as a laboratory reagent.

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2 protocols using orthophosphoric acid

Quantification of Catechins and Related Compounds

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Standards for catechins and related compounds were used in this work. Catechins and other related compounds were purchased from Sigma-Aldrich (St Louis, Missouri, MO, USA): (+)-catechin (C), (−)-gallocatechin (GC), (−)-epigallocatechin (EGC), (−)-epicatechin (EC), (−)-epigallocatechin gallate (EGCG), (−)-gallocatechin gallate (GCG), (−)-epicatechin gallate (ECG), gallic acid (GA), and caffeine (Caf.). HPLC-grade acetonitrile and methanol, including ethyl acetate, were supplied by Merck (Darmstadt, Germany). Analytical-grade acetic acid (Sigma-Aldrich) and orthophosphoric acid (BDH, Poole, U.K.) were also purchased. HPLC-grade water (18 MΩ) was prepared using a Millipore Milli-Q purification system (Millipore Corp. Bedford, MA, USA) and used to prepare all solutions.

Wangkarn S., Grudpan K., Khanongnuch C., Pattananandecha T., Apichai S, & Saenjum C. (2021). Development of HPLC Method for Catechins and Related Compounds Determination and Standardization in Miang (Traditional Lanna Fermented Tea Leaf in Northern Thailand). Molecules, 26(19), 6052.

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Synthesis and Characterization of Electrochemical Reagents

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Lithium chloride (97%), metformin hydrochloride (97%), phenyl biguanide hydrochloride (98%), phenformin hydrochloride (97%), tetraethylammonium chloride (TEA + , 99%), sodium phosphate monobasic monohydrate (98%), and triethylamine (99.5%), bis(triphenylphosphoranylidene) ammonium chloride (BTPPA + Cl -, 97%) and potassium tetrakis(4-chlorophenylborate) (K + TPBCl -, 98%)
were purchased from Sigma Aldrich. Tetramethylammonium chloride (TMA + , 99%) was purchased from Fluka BioChemika. Sodium hydroxide (97%), ortho-phosphoric acid (85%), both from BDH Prolabo and hydrochloric acid (Merck, 37%) were used to adjust pH. All aqueous solutions were prepared in ultrapure water (18.2 M cm, Elga Water). HPLC-grade acetonitrile and 1, 2-dichloroethane (DCE)
were purchased from Sigma Aldrich. BTPPA + TPBCl -and TMA + TPBCl -were prepared by metathesis and dissolved in DCE according to the published procedure. 40

Jajuli M.N., Hussin M.H., Saad B., Rahim A.A., Hébrant M, & Herzog G. (2019). Electrochemically Modulated Liquid-Liquid Extraction for Sample Enrichment. Analytical chemistry, 91(11).

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