We made SEC-MALLS measurements on an HPLC system equipped with three straight connected columns: TSKgel G6000PWXL, G5000PWXL, and G4000PWXL (7.5 mm i.d. × 600 mm, Tosoh Corporation, Tokyo, Japan). The column outlet was connected to a Dawn Heleos-II multiangle laser light scattering photometer (Wyatt Technology Corp., Santa Barbara, USA) (λ = 658 nm) which was connected to an Optilab rEX differential refractometer (Wyatt Technology Corp.). The mobile phase was 0.05 M Na2SO4/0.01 M EDTA (pH 6). The flow rate was 0.7 mL/min. The injection volume was 100 μL, and the sample concentration was 0.2 or 0.4 % for appropriate light scattering intensity. The sample solution was filtered with a 0.22 μm filter before injection. The value of the refractive index increment (δn/δc) for the alginate was 0.150 mL/g, according to a previous report.13) (link) Data from the light scattering and the differential refractometers were collected and processed using Astra (v. 5.3.4.14) software (Wyatt Technology Corp.).
Astra v 5.3.4.14 software
Manufactured by Wyatt Technology
Astra (v. 5.3.4.14) is a software product developed by Wyatt Technology. It serves as a data analysis and processing tool for laboratory equipment.
Automatically generated - may contain errors
Lab products found in correlation
Dawn heleos 2 multi angle laser light scattering photometer, by Wyatt Technology (1 mentions)
Optilab rex differential refractometer, by Wyatt Technology (1 mentions)
Dawn heleos 2 multiangle laser, by Wyatt Technology (1 mentions)
Ohpak sb 805 hq, by Resonac (1 mentions)
Ohpak sb 806 hq, by Resonac (1 mentions)
Optilab rex, by Wyatt Technology (1 mentions)
2 protocols using astra v 5.3.4.14 software
1
Alginate Characterization by SEC-MALLS
2
SEC-MALS Analysis of Purified PGA
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SEC-MALS measurements were performed using an HPLC system equipped with three straight connected columns: OHpak SB-807HQ, OHpak SB-806HQ, and OHpak SB-805HQ (Showa Denko, Tokyo, Japan). Purified PGA samples were dissolved in deionized water at a final concentration of 1 mg mL -1 unless stated otherwise. The column outlet was connected to a Dawn Heleos-II multiangle laser light scattering photometer (Wyatt Technology Corp., Santa Barbara, CA, USA) (λ = 658 nm), which was connected to an Optilab rEX differential refractometer (Wyatt Technology Corp.). Prior to analysis, the samples were filtered through polyethersulfone syringe filters with a pore size of 0.45 µm (ADVANTEC, Tokyo, Japan). The analysis conditions were as follows: isocratic elution with 200 mmol L -1 KNO3 at 40°C; flow rate, 0.7 mL min -1 ; and PGA sample injection volume, 50 µL. Data from the light scattering and differential refractometers were collected and processed using Astra (v. 5.3.4.14) software (Wyatt Technology Corp.) [16] .
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