Western blotting was performed by standard methods.49 (link) Cells were lysed in NP40 solution supplemented with 1mM Na3VO4, 1mM PMSF containing the protease inhibitor cocktail. Protein concentrations of lysates were calculated by the BCA (Bicinchoninic Acid) assay. Protein (approximately 30 μg) was diluted in a sample buffer [2.5% SDS, 10% glycerol, 5% β-mercapto-ethanol, 50mM Tris (pH = 6.8) and 0.1% bromophenol blue] and subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). A monoclonal rabbit anti-human anti-MC1R (Abcam) was diluted at 1:2000. β-Actin (1:10000, cat. # A5441, SIGMA) was utilized as a control for normalization of protein gel loading. Detection of proteins was done with peroxidase-conjugated anti-mouse (cat. # 7076S, Cell Signaling, 1:5000) or anti-rabbit IgG secondary antibodies (cat. # 7074S, Cell Signaling, 1:2000) and ECL (PerkinElmer).
Anti human anti mc1r
Manufactured by Abcam
Anti-human anti-MC1R is a laboratory equipment product. It is an antibody that specifically binds to the human melanocortin 1 receptor (MC1R) protein. MC1R is a G protein-coupled receptor involved in the regulation of pigmentation. The antibody can be used for the detection and study of MC1R in various experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti human anti mc1r
1
Western Blotting of MC1R Protein
2
Western Blotting for Melanoma Biomarker
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Western blotting was performed by standard methods. 49 (link) Cells were lysed in NP40 solution supplemented with 1mM Na 3 VO 4 , 1mM PMSF containing the protease inhibitor cocktail. Protein concentrations of lysates were calculated by the BCA (Bicinchoninic Acid) assay. Protein (approximately 30 μg) was diluted in a sample buffer [2.5% SDS, 10% glycerol, 5% β-mercapto-ethanol, 50mM Tris (pH = 6.8) and 0.1% bromophenol blue] and subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). A monoclonal rabbit antihuman anti-MC1R (Abcam) was diluted at 1:2000. β-Actin (1:10000, cat. # A5441, SIGMA) was utilized as a control for normalization of protein gel loading. Detection of proteins was done with peroxidase-conjugated antimouse (cat. # 7076S, Cell Signaling, 1:5000) or anti-rabbit IgG secondary antibodies (cat. # 7074S, Cell Signaling, 1:2000) and ECL (PerkinElmer).
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