Serum samples were taken in the morning following an overnight fast and stored at -80°C until analysis. Serum tOC was measured using an automated immunoassay (Elecsys 170; Roche Diagnostics) [29 (link)]. Serum ucOC was measured using the hydroxyapatite binding method, a commonly used, well established method [30 (link)]. Each sample was measured once and the inter-assay coefficients of variation were 5.4% and 9.2% for tOC and ucOC, respectively.
Elecsys 170
Manufactured by Roche
Sourced in United States, Switzerland
The Elecsys 170 is an automated immunoassay analyzer designed for clinical laboratories. It is capable of performing a variety of immunoassay tests, including those for hormones, tumor markers, and infectious disease markers. The Elecsys 170 is able to process multiple samples simultaneously and provides rapid, accurate results.
Automatically generated - may contain errors
6 protocols using elecsys 170
1
Serum osteocalcin measurement protocol
2
Serum Iron Index Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SII is obtained by calculating the total peripheral platelet count × neutrophil-to-lymphocyte ratio (N/L) (SII = P × N/L). Lymphocyte, neutrophil, and platelets counts were measured by complete blood count using automated hematology analyzing devices (Coulter DxH 800 analyzer) and present as ×103 cells/ul. Serum ferritin (ng/ml) is derived from laboratory data and serum ferritin concentrations from 2015-2016 are measured with a Roche Elecsys-170 clinical analyzer, while serum ferritin concentrations from 2017-2018 are measured with a Roche Cobas e601 clinical analyzer (Roche Diagnostics, Indianapolis, USA). We grouped serum ferritin into three groups according to the WHO guideline definition of serum iron status: men and women with serum ferritin < 15 ug/L were defined as iron deficiency; Women with serum ferritin > 150 ug/L and men with serum ferritin >200 ug/L are defined as iron overload; Other serum ferritin levels are considered normal iron status [24].
3
Serum Total and Uncarboxylated Osteocalcin Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum tOC will be analyzed as described previously [9 (link),10 (link),19 (link),39 (link)]. In brief, tOC will be measured using an automated immunoassay (Elecsys 170; Roche Diagnostics). Serum ucOC will be measured by the same immunoassay after absorption of cOC on 5 mg/mL hydroxyl-apatite slurry, following the method described by Gundberg et al [77 (link)].
4
Biomarker Measurement in NHANES
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Following the primary screening questionnaire, all participants who satisfied the inclusion criteria were invited to the mobile examination center (MEC) for further measurement. Physiological measurements, specimen collection, and laboratory tests were performed by professional medical staff according to MEC laboratory procedures manual. Serum iron, ferritin, TFR, ALT, aspartate aminotransferase (AST) and γ-glutamyl transferase (GGT) were measured using the refrigerated serum samples. Serum iron was measured by collaborative laboratory services using timed-endpoint method. ALT, AST, and GGT were measured using Beckman UniCel DxC800 Synchron by the same laboratory. Ferritin was measured using Roche Elecsys-170, and the method for TFR is immuno-turbidimetry using Roche kits on the Hitachi 912 chemistry analyzer (2003–2008) or Hitachi Mod P clinical analyzer (2009–2010) or Cobas C501 clinical analyzer (2015–2018). Whole blood lead (Pb) and cadmium (Cd) content were measured using mass spectrometry. Detailed instructions on sample collection and other methodological approaches can be found at https://wwwn.cdc.gov/nchs/nhanes/AnalyticGuidelines.aspx .
5
Serum Cortisol Concentration Assessment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Frozen supernatants of the blood were sent to the Center Hospital of Tai’an city for total cortisol concentration assessment using electrochemical luminescence immunoassay with modular analytics immunoassay analyzer (Elecsys 170, Roche Diagnostics, Basel, Switzerland).
6
Serum Osteocalcin Biomarkers Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum osteocalcin and undercarboxylated osteocalcin: Total serum OC was measured using an automated immunoassay (Elecsys 170; Roche Diagnostics). This assay has a sensitivity of 0.5μg•L -1 , with an intra-assay precision of 1.3%. Serum ucOC was measured by the same immunoassay after adsorption of carboxylated OC on 5 mg/mL hydroxyl-apatite slurry, following the method described by Gundberg et al. [24] β-CTX and P1NP: β-isomerized C-terminal telopeptide (β-CTX, bone resorption marker) and procollagen I N-terminal propeptide (P1NP, a bone formation marker) were analysed at Austin pathology, Melbourne using a Roche Hitachi Cobas e602 immunoassay analyser, according to the manufacturer's guidelines.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!