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Cd146 alexa488

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CD146-Alexa488 is a fluorescently-labeled antibody that recognizes the CD146 cell surface protein. It can be used for the detection and analysis of cells expressing CD146 in various applications.

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Lab products found in correlation

Cd133 pe, by BioLegend (2 mentions) Cd34 alexa647, by BD (2 mentions)

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CD146 (54 citations)

2 protocols using cd146 alexa488

1

Quantification of CD34+CD133+CD146+ Hematopoietic Stem Cells

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BMCs were harvested freshly from mice for the experiment and cultured in DMEM high glucose media with 10% FBS. 100 ng/ml of SCF, tmSCF, tmSCFPLs, tmSCFNDs were added to the BMCs media, and cells were incubated for 30 min. Treatment was stopped by placing the cell plate on ice. The cells were stained for CD34-Alexa647 (BD Biosciences), CD133-PE (BioLegend), and CD146-Alexa488 (BD Biosciences). CD34-CD133+CD146+ cells are quantified by FlowJo software. Gating was performed similarly to the analysis of peripheral blood.
Takematsu E., Massidda M., Auster J., Chen P.C., Im B., Srinath S., Canga S., Singh A., Majid M., Sherman M., Dunn A., Graham A., Martin P, & Baker A.B. (2022). Transmembrane stem cell factor protein therapeutics enhance revascularization in ischemia without mast cell activation. Nature Communications, 13, 2497.
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2

Quantifying EPC Mobilization Treatments

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To measure the ability of treatments to induce EPCs to peripheral blood, 240 µg/kg of SCF, tmSCF, tmSCFPLs, and tmSCFNDs were injected subcutaneously for consecutive four days. At the end of day 4, peripheral blood was collected for EPC marker analysis. As EPCs markers, FLK1, CD146, CD34, and CD133 were used for flow cytometry analysis. Then cells are stained for CD34-Alexa647 (BD Biosciences), CD133-PE (BioLegend), CD146-Alexa488 (BD Biosciences), and Flk-1-APC-Cy7 (BD Biosciences). An example of the gating strategy used for the studies is shown in Supplementary Fig. 14.
Takematsu E., Massidda M., Auster J., Chen P.C., Im B., Srinath S., Canga S., Singh A., Majid M., Sherman M., Dunn A., Graham A., Martin P, & Baker A.B. (2022). Transmembrane stem cell factor protein therapeutics enhance revascularization in ischemia without mast cell activation. Nature Communications, 13, 2497.
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