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Anti phospho pkc antibodies

Manufactured by Cell Signaling Technology
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Anti-phospho-PKC antibodies are tools used to detect and study the phosphorylation of protein kinase C (PKC) enzymes. These antibodies specifically recognize the phosphorylated forms of PKC, allowing researchers to monitor the activation and regulation of this important signaling pathway.

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Lab products found in correlation

Anti phospho rps6kb1 antibodies, by Cell Signaling Technology (2 mentions) Caprylic acid, by Merck Group (1 mentions) Glibenclamide, by Merck Group (1 mentions) Calmidazolium, by Merck Group (1 mentions) Genistein, by Merck Group (1 mentions) Gf109203x, by Merck Group (1 mentions) L name, by Merck Group (1 mentions) Levcromakalim, by Bio-Techne (1 mentions) L arginine, by Merck Group (1 mentions) Indomethacin, by Merck Group (1 mentions) Methylene blue, by Merck Group (1 mentions)

3 protocols using anti phospho pkc antibodies

1

Vasodilation Mechanisms in Rat Aorta

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Lipofundin MCT/LCT and Intralipid were supplied by B. Brown Korea (Seoul, Korea) and Fresenius Kabi Korea (Seoul, Korea), respectively. L-arginine, L-NAME, glibenclamide, ODQ, methylene blue, calmidazolium, indomethacin, caprylic acid, GF109203X and genistein were obtained from Sigma-Aldrich (St Louis, MO, USA). Levcromakalim was purchased from Tocris Bioscience (Bristol, United Kingdom). The anti-phospho-eNOS (at Ser1177), anti-eNOS, and anti-phospho-PKC antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-PKC antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). All the chemical concentrations are expressed as the final organ bath concentration. Levcromakalim was dissolved in ethanol (final concentration: 0.19%). glibenclamide, ODQ, calmidazolium, indomethacin and GF109203X were dissolved in dimethyl sulfoxide (final concentration of dimethyl sulfoxide: less than 0.1%). All other chemicals were dissolved in distilled water.
Lee S.H., Kang D., Ok S.H., Kim J.Y., Bae S.I., Hwang Y., Park K.E., Kim J.W, & Sohn J.T. (2020). Lipofundin MCT/LCT Inhibits Levcromakalim-Induced Vasodilation by Inhibiting Endothelial Nitric Oxide Release. International Journal of Molecular Sciences, 21(5), 1763.
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2

Caffeine Modulates PKB Phosphorylation

Cited 2 times
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Differentiated Dictyostelium cells were incubated in DB with 2 mM caffeine for 20 min at 22°C, washed twice with ice-cold DB, and resuspended at 5×107 cells/ml. Cells were shaken at 200 rpm at 22°C and stimulated with 1 μM cAMP 39 (link). Aliquots were taken at different time points and lysed in SDS-PAGE sample buffer. Proteins were analyzed by immunoblotting with antibodies to PKBA and PKBR1. As extensively described in many studies 23 (link), phosphorylation of PKBA and PKBR1 were detected using anti-phospho-RPS6KB1 antibodies (Cell Signaling, 9205) and anti-phospho-PKC antibodies (Cell Signaling, 2060) because of the similar amino acid sequences around the phosphorylation sites (Fig. S4).
Senoo H., Kamimura Y., Kimura R., Nakajima A., Sawai S., Sesaki H, & Iijima M. (2019). Phosphorylated Rho-GDP Directly Activates mTORC2 Kinase Toward AKT Through Dimerization with Ras-GTP to Regulate Cell Migration. Nature cell biology, 21(7), 867-878.
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3

Caffeine Modulates PKB Phosphorylation

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Differentiated Dictyostelium cells were incubated in DB with 2 mM caffeine for 20 min at 22°C, washed twice with ice-cold DB, and resuspended at 5×107 cells/ml. Cells were shaken at 200 rpm at 22°C and stimulated with 1 μM cAMP 39 (link). Aliquots were taken at different time points and lysed in SDS-PAGE sample buffer. Proteins were analyzed by immunoblotting with antibodies to PKBA and PKBR1. As extensively described in many studies 23 (link), phosphorylation of PKBA and PKBR1 were detected using anti-phospho-RPS6KB1 antibodies (Cell Signaling, 9205) and anti-phospho-PKC antibodies (Cell Signaling, 2060) because of the similar amino acid sequences around the phosphorylation sites (Fig. S4).
Senoo H., Kamimura Y., Kimura R., Nakajima A., Sawai S., Sesaki H, & Iijima M. (2019). Phosphorylated Rho-GDP Directly Activates mTORC2 Kinase Toward AKT Through Dimerization with Ras-GTP to Regulate Cell Migration. Nature cell biology, 21(7), 867-878.
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