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Hs peg5k nh2 hcl salt

Manufactured by Merck Group

HS-PEG5K-NH2 (HCl salt) is a polyethylene glycol (PEG) derivative with a terminal amino group. It is commonly used as a linker or spacer molecule in various biotechnological and pharmaceutical applications.

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2 protocols using hs peg5k nh2 hcl salt

1

Urease-Functionalized Magnetic Beads

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Mono Mag magnetic beads 0.5 μm (Ocean
NanoTech), glutaraldehyde grade II (Glu, 25% in H2O), HS-PEG5K-NH2 (HCl salt), urease (from Canavalia ensiformis, Type IX, powder, 50 000–100 000 units g/g solid),
urease activity kit, urea (99.9%), and urease (from Canavalia ensiformis, Type IX, powder, 50 000–100
000 units/g solid) were obtained from Sigma-Aldrich. Red fluorescent
amine-modified beads 0.2 μm (2% solids), PBS, Pierce BCA protein
assay kit, and Dulbecco’s modified Eagle’s medium (DMEM)
were purchased from ThermoFischer Scientific. Standard gray V4 resin
(FLGPGR04) for the 3D printing of rigid molds was purchased at FormLabs.
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2

Fabrication of Silica Nanocomposite Biosensors

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Ethanol (99%), mEthanol (99%), hydrochloric acid (37% in water), ammonium hydroxide (25% in water), tetraethylorthosilicate (TEOS; 99%), triEthanolamine (TEOA; 99%), cetyltrimethylammonium bromide (CTAB; 99%), 3-APTES (99%), GA (25% in water), urease (from Canavalia ensiformis, type IX, powder, 50,000 to 100,000 U/g of solid), urea (99.9%), BSA (lyophilized powder) gold(III) chloride trihydrate (HauCl4), sodium citrate (NaCit), and HS-PEG5K-NH2 (HCl salt) were purchased from Sigma-Aldrich. PBSs were purchased from Thermo Fisher Scientific. Standard Grey V4 resin (FLGPGR04) for the 3D printing of rigid molds was purchased at FormLabs. SYLGARD 184 Silicone Elastomer was purchased from Ellsworth Adhesives Ibérica.
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