Log In Sign up
The largest database of trusted experimental protocols

Tinyarray imager

Manufactured by Grace Bio-Labs
Visit Supplier

The TinyArray imager is a compact and versatile instrument designed for high-quality fluorescence imaging of small sample formats. Its core function is to capture and analyze fluorescent signals from a variety of sample types, including microplates, slides, and other miniaturized formats.

Automatically generated - may contain errors

Lab products found in correlation

Mapix, by Innopsys (2 mentions) Arraycam system, by Grace Bio-Labs (2 mentions)

2 protocols using tinyarray imager

1

Quantitative Microarray Image Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
To enable quantification of the dye concentration indicative of the antibody concentration in all spots of the array, the intensity must be determined in each spot. For this purpose, microarray slides were printed with a distinct pattern of 18 × 18 dots (150 μm diameter, 280 μm spacing). The slides were imaged using both the TinyArray imager and the commercial ArrayCam system (Grace Bio-Labs, Bend, OR). As the size and distances of the spots were known, the background-subtracted median spot fluorescence intensity was measured and quantified using Mapix (Innopsys) as previously described.25–27 (link) Mean fluorescence intensity (MFI) of the four replicate spots for each antigen was utilized for antigen heat map generation and data analysis.
Hedde P.N., Abram T.J., Jain A., Nakajima R., Ramiro de Assis R., Pearce T., Jasinskas A., Toosky M.N., Khan S., Felgner P.L., Gratton E, & Zhao W. (2020). A modular microarray imaging system for highly specific COVID-19 antibody testing. Lab on a Chip, 20(18), 3302-3309.
+ Open protocol
+ Expand
2

Quantifying Antibody Concentrations in Microarray

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
To enable quantification of the dye concentration indicative of the antibody concentration in all spots of the array, the intensity must be determined in each spot. For this purpose, microarray slides were printed with a distinct pattern of 18 × 18 dots (150 μm diameter, 280 μm spacing). The slides were imaged using both the TinyArray imager and the commercial ArrayCam system (Grace Bio-Labs, Bend, OR). As the size and distances of the spots were known, the background-subtracted median spot fluorescence intensity was measured and quantified using Mapix (Innopsys) as previously described22 (link)–24 (link). Mean fluorescence intensity (MFI) of the four replicate spots for each antigen was utilized for antigen heat map generation and data analysis.
Hedde P.N., Abram T.J., Jain A., Nakajima R., de Assis R.R., Pearce T., Jasinskas A., Toosky M.N., Khan S., Felgner P.L., Gratton E, & Zhao W. (2020). A Modular Microarray Imaging System for Highly Specific COVID-19 Antibody Testing. bioRxiv.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!