Three antibody clones against Zap‐70 (SBZAP, 2F3.2, and 1E7.2) were tested for flow cytometry and Western blot. Anti‐Zap‐70‐PE (SBZAP) was further used for flow cytometry. Cells were fixed with 1% paraformaldehyde (Merck, Germany) for 5 min, washed once with PBS, and permeabilized with 1X Perm II reagent (BD, USA) according to the manufacturer's instructions. The staining panel included either anti‐CD3‐FITC (clone HIT3a, BioLegend), anti‐Zap‐70‐PE (clone SBZAP, Beckman Coulter) and anti‐CD22‐APC (clone S‐HCL‐1, BD), or anti‐CD3‐PE‐Cy7 (clone UCHT1, BioLegend), anti‐CD19‐FITC (clone HIB19, eBioscience), anti‐Zap‐70‐PE (clone SBZAP, Beckman Coulter), and anti‐CD22‐APC (clone S‐HCL‐1, BD).
Anti cd22 apc clone s hcl 1
Manufactured by BD
Anti-CD22-APC (clone S-HCL-1) is a laboratory reagent used for the detection and analysis of CD22-expressing cells. It is a monoclonal antibody conjugated with the fluorescent dye allophycocyanin (APC). This product is intended for research use only and its core function is to provide a tool for the identification and characterization of CD22-positive cells.
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Lab products found in correlation
1 protocol using anti cd22 apc clone s hcl 1
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Zap-70 Protein Expression Analysis
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