Ethidium bromide etbr solution

Manufactured by Merck Group

Sourced in United States

Ethidium bromide (EtBr) solution is a laboratory reagent commonly used in molecular biology and genetics research. It is a fluorescent dye that intercalates with nucleic acids, such as DNA and RNA, and emits a reddish-orange fluorescence when exposed to ultraviolet light. EtBr solution is primarily used for the visualization and detection of nucleic acids in various applications, including agarose gel electrophoresis and DNA staining.

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2 protocols using ethidium bromide etbr solution

Asiatic Acid and Oxidative Stress Modulation

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Asiatic acid (AsA), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), N-Acetyl cysteine (NAC), 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA), Acridine orange, ethidium bromide (EtBr) solution, Trypan blue, and capase-3 inhibitors (Z-DEVD-FMK) were obtained from Sigma, St. Louis, MO, USA. Roswell Park Memorial Institute (RPMI 1640) and antibiotic-antimycotic solution were purchased from Himedia, Pune, India, whereas fetal bovine serum (FBS) used during in vitro culture was obtained from Gibco, West Chester, PA, USA. All the real-time primers employed in the study were synthesized and procured from Integrated DNA Technologies (IDT), Coralville, IA, USA. Verso cDNA synthesis kit and DyNAmoColorFlash SYBR Green qPCR Kit were obtained from Thermo-Scientific, Waltham, MA, USA.

Alafnan A., Hussain T., Rizvi S.M., Moin A, & Alamri A. (2021). Prostate Apoptotic Induction and NFκB Suppression by Dammarolic Acid: Mechanistic Insight into Onco-Therapeutic Action of an Aglycone Asiaticoside. Current Issues in Molecular Biology, 43(2), 932-940.

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Synthesis of Fluorescent Nanoparticles for Immunoassays

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All synthesis procedures were conducted using ultrapure water obtained from a Milli-Q system (Millipore Corporation, Billerica, MA) with resistivity of 18.2 MΩ·cm. Potassium hexacyanoferrate (II) trihydrate (MW 422.39; K₄[Fe(CN)₆]·3H₂O), iron (III) chloride hexahydrate (MW 270.3; Fe(Cl)₃·6H₂O), and citric acid were purchased from Sigma-Aldrich (St. Louis, MO, USA), and were used as supplied. Acetone, ethanol, and ethidium bromide (EtBr) solution were obtained from Sigma-Aldrich. Poly(ethylenimine) (PEI, Mw 2,000, Mn 1,800, 50% w/v in H₂O) was purchased from Sigma-Aldrich, and diluted in acetate buffer (pH 5.2, Sigma-Aldrich). Murine CpG oligodeoxynucleotide (CpG) TLR9 ligand (ODN 1585; Class A)) was purchased from InVivoGen (San Diego, CA, USA). Fluorescent antibodies against HMGB1 and calreticulin were purchased from Abcam (Cambridge, UK). Lipopolysaccharide (LPS) was purchased from Thermo Fisher Scientific (Carlsbad, CA, USA).

Cano-Mejia J., Bookstaver M.L., Sweeney E.E., Jewell C.M, & Fernandes R. (2019). Prussian blue nanoparticles-based antigenicity and adjuvanticity trigger robust antitumor immune responses against neuroblastoma. Biomaterials science, 7(5), 1875-1887.

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