13c nahco3

Manufactured by Cambridge Isotopes

13C-NaHCO3 is a stable isotope-labeled compound consisting of sodium bicarbonate (NaHCO3) with the carbon-13 (13C) isotope. It is a white, crystalline powder used in various research and analytical applications.

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2 protocols using 13c nahco3

Metabolic Pathway Analysis with Isotopic Labeling

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The following reagents were obtained from Sigma-Aldrich: glucose, 1,3-propanediol, phenylboronic acid, cycloheximide, 23BD, acetoin, pyruvate, PEP, 6PG, G6P, F6P, Ru5P, R15P, ATP, dithiothreitol (DTT), NADH, NADPH, NADP⁺, phosphocreatine, RuBisCO, pyruvate kinase, lactate dehydrogenase, G6P dehydrogenase, 3PGA kinase, GAPDH and creatine kinase. U-¹³C glucose and ¹³C-NaHCO₃ were obtained from Cambridge Isotope Laboratories. IPTG and chloramphenicol were obtained from Fischer Scientific. Gentamycin was purchased from Teknova. Spectinomycin was purchased from MP Biomedicals. Kanamycin was purchased from IBI Scientific. Phusion polymerase was purchased from New England Biolabs. All oligonucleotide synthesis and DNA sequencing were performed by Eurofins MWG Operon Inc.

Kanno M., Carroll A.L, & Atsumi S. (2017). Global metabolic rewiring for improved CO2 fixation and chemical production in cyanobacteria. Nature Communications, 8, 14724.

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C-Labeling of Bacterial Cultures

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Cultures were grown in four replicates in 2-L flasks containing 1.5-L medium. For ¹³C labeling, ¹³C-NaHCO₃ (Cambridge Isotope Laboratories) was added to a final concentration of 0.174 g l^− 1 to flasks No.1, No.2, No.3, and No.4 at D1, D3, D5, and D7, respectively. Samples were collected at 1 day after labeling (D2, D4, D6, and D8) as described above.

Huang A., Wu S., Gu W., Li Y., Xie X, & Wang G. (2019). Provision of carbon skeleton for lipid synthesis from the breakdown of intracellular protein and soluble sugar in Phaeodactylum tricornutum under high CO2. BMC Biotechnology, 19, 53.

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