Several previously described growth media were used to cultivate the AOM used in this study. A comprehensive guide with medium components and cultivation conditions is provided in the Supplementary Materials and Methods , Tables S1 , and S2 . Briefly, all cultures were grown without shaking, in the dark, at their optimum growth temperature and pH, unless otherwise stated. Ammonium (NH4Cl) from pre-sterilized stocks was added as substrate as needed. The growth medium of Nitrosarchaeum koreense MY1, ‘Ca. Nitrosotenuis chungbukensis’ MY2, ‘Ca. Nitrosotenuis uzonensis’ N4, N. maritimus SCM1, Nitrosopumilus piranensis D3C, and Nitrosopumilus adriaticus NF5 was supplemented with sodium pyruvate (0.5 mM) at all times. The pH of all growth media were adjusted when necessary by addition of sterile NaHCO3. Ammonia oxidation activity was determined by measuring ammonium, nitrite, and nitrate concentrations photometrically [36 –39 (link)] using an Infinite 200 Pro M Nano+ spectrophotometer (Tecan Group AG, Switzerland).
Infinite 200 pro m nano spectrophotometer
Manufactured by Tecan
Sourced in Switzerland
The Infinite 200 Pro M Nano+ spectrophotometer is a lab equipment product manufactured by Tecan. It is designed to measure the absorbance of small sample volumes with high accuracy. The core function of this device is to quantify the concentration of nucleic acids and proteins in microvolume samples.
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Lab products found in correlation
2 protocols using infinite 200 pro m nano spectrophotometer
1
Cultivation and Ammonia Oxidation Assays for Archaeal Ammonia Oxidizers
2
Quantitative VEGF-A and IL-6 Measurement
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The concentration of VEGF-A or IL-6 in cell culture supernatants of the treated MIO-M1 cells were determined using a Quantikine human VEGF-A ELISA kit (cat. no. DVE00, Bio-Techne) and a Quantikine Human IL-6 ELISA kit (cat. no. DVE6050, Bio-Techne), respectively. For measuring IL-6 levels, samples were diluted 1:10 in PBS without Ca2+- and Mg2+-ions (cat. no. 14190-169, Thermo Fisher Scientific, Inc.), and undiluted samples were used to determine the concentration of VEGF-A. Duplicate samples were processed according to the manufacturer's instructions and the analyte-dependent absorbance at 450 nm (reference wavelength: 570 nm) was measured 15 min after the addition of the stop solution with an Infinite 200Pro M Nano spectrophotometer controlled by Tecan I control software (version 2.0.10.0; Tecan Group, Ltd.). Standard curves (16 pg/ml to 1 ng/ml VEGF-A or 3-300 pg/ml IL-6) were always generated in parallel to the analysis of samples.
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