Ct a199

Manufactured by Chemietek

The CT-A199 is a laboratory equipment product designed for general laboratory applications. It serves as a core instrument for various experimental and analytical procedures. The product's primary function is to provide a controlled environment for various laboratory tasks. No further details or interpretation on the intended use of this product are provided.

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Lab products found in correlation

2 protocols using ct a199

Targeted Cancer Therapy Compounds and Antibodies

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The following drugs were purchased: Dinaciclib (SCH727965) for in vitro and in vivo studies (S2768; Selleckchem), lapatinib ditosylate (Tykerb) for in vitro and in vivo studies (M1802; Abmole), neratinib for in vivo studies (M1913; Abmole), A-1210477 (CT-A121; Chemietek), A-1331852 (22963; Cayman Chemicals), tucatinib (HY-16069; Medchem), and ABT-199 (venetoclax) (CT-A199; Chemietek). The antibodies used in this study were as follows: anti-Bak (AB-1 clone for IP) (AM03; EMD Millipore), anti-Bak (3814S; Cell Signaling), anti-Bim (C34C5) (2933S; Cell Signaling), anti–BCL-xL (54H6) (2764S; Cell Signaling), anti–Bcl-2 (D55G8) (Human Specific) (4223S; Cell Signaling), anti-cleaved PARP (Asp214) (D64E10) (5625S; Cell Signaling), anti-GAPDH (6C5) (sc-32233; Santa Cruz), anti–MCL-1 (S-19) (sc-819; Santa Cruz), anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) (4370S; Cell Signaling), anti-phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) (5364S; Cell Signaling), anti-phospho-Akt (Thr308) (244F9) (4056S; Cell Signaling), anti-phospho-Akt (Ser473) (D9E) (4060S; Cell Signaling), anti-HER2/ErbB2 (29D8) (2165S; Cell Signaling), anti-phospho-HER2/ErbB2 (Tyr1248) (2247S; Cell Signaling), anti-phospho-Rpb1 CTD (Ser 2/5) (4375S; Cell Signaling), Normal Rabbit IgG for IP (sc-2027; Santa Cruz), and Normal Mouse IgG for IP (sc-2025; Santa Cruz).

Floros K.V., Jacob S., Kurupi R., Fairchild C.K., Hu B., Puchalapalli M., E. Koblinski J., Dozmorov M.G., Boikos S.A., Scaltriti M, & Faber A.C. (2021). Targeting transcription of MCL-1 sensitizes HER2-amplified breast cancers to HER2 inhibitors. Cell Death & Disease, 12(2), 179.

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Preparation of BCL-2 inhibitor stocks

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Venetoclax (>99.9%, Chemietek CT-A199), A1155463 (99.5%, Chemietek CT-A115) and NVP-CGM097 (100% optically pure, Chemitek CT-CGM097), were directly used without further purification. Venetoclax, A1155463, and NVP-CGM097 were each dissolved in DMSO as 10 mM stocks. Stocks were aliquoted and stored at −20 °C until use.

Shui S., Gainza P., Scheller L., Yang C., Kurumida Y., Rosset S., Georgeon S., Di Roberto R.B., Castellanos-Rueda R., Reddy S.T, & Correia B.E. (2021). A rational blueprint for the design of chemically-controlled protein switches. Nature Communications, 12, 5754.

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