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Bioplex luminex

Manufactured by Bio-Rad
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The BioPlex Luminex is a multiplex assay system that enables the simultaneous detection and quantification of multiple analytes from a single sample. It utilizes Luminex xMAP technology, which is based on color-coded magnetic beads coated with capture antibodies. The system measures the fluorescent signals emitted by the beads to provide accurate and sensitive data on the levels of various biomolecules in the sample.

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Lab products found in correlation

Duoset elisa, by R&D Systems (1 mentions) Elisa assays, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

BioPlex 200 Luminex bead array reader (3 citations) Luminex Bioplex 200 analyzer (3 citations) BioPlex Luminex platform (3 citations) Luminex/Bioplex assay 200 system (2 citations)

4 protocols using bioplex luminex

1

Multiplex Biomarker Analysis of MMP and TIMP Levels

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Firstly, as a preliminary study 9 MMPs (-1, -2, -3, -7, -8, -9, -10, -12, -13), TIMP-1, and TIMP-2 plasma levels were measured in non-obese and people with overweight and obesity by BioPlex Luminex (BioRad; 2 lasers, 3 colors, High-Throughput System for multiplex cytometric bead arrays). From the preliminary results given by this technique we have chosen to focus on MMP (-1, -2, -3 and -9) and TIMP-1 and TIMP-2. Then, MMP (-1, -2, -3 and -9) and TIMP-1 and TIMP-2 levels were determined by Enzyme-Linked Immunosorbent Assays (ELISA) using commercially available kits, human MMP-1 (DYS901), human MMP-2 (DY902), human MMP-3 (DY513), human MMP-9 (DY911), human TIMP-1 (DY970) and human TIMP-2 (DY971) (DUOSET®ELISA, R&D systems), respectively, according to the manufacturer's instructions.
Boumiza S., Chahed K., Tabka Z., Jacob M.P., Norel X, & Ozen G. (2021). MMPs and TIMPs levels are correlated with anthropometric parameters, blood pressure, and endothelial function in obesity. Scientific Reports, 11, 20052.
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2

Multiplex ELISA Analysis of Cytokines

Cited 1 time
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Cytokine levels in total pooled plasma and organ tissue homogenates from
each group were first determined by a multiplex ELISA
(eBiosciences-Affymetrix/ThermoFisher) analyzing 36 cytokines per sample. Data
was analyzed using a BioPlex Luminex plate reader (BioRad) in our Genomics core
facility. Pooled samples were used as a cost effective approach to identify to
systemic cytokine changes (20 (link)–22 (link)). This method
of screening 36 cytokines allowed us to identify the molecules that differed
between wild-type and DBP deficient animals, and subsequently to customize a
multiplex ELISA (eBiosciences-Affymetrix/ThermoFisher) for analyzing 12 key
cytokines: CCL2 (MCP-1), CCL3 (MIP-1α), CCL4 (MIP-1β), CCL5
(RANTES), CXCL1 (GRO-α), CXCL2 (MIP-2), CXCL5 (ENA-78), CXCL10 (IP-10),
G-CSF (CSF-3), IL-2, IL-6 and IL-10. These cytokines were measured in samples
from both individual mice and three different pools in each of the four
experimental groups. To assure the validity of the data, each sample was
measured three times: 36-plex of pooled samples as a screen, 12-plex of
individual samples, 12-plex of three different pools. Results reported herein
are those from a 12-plex of individual samples or pooled samples.
Kew R.R., Tabrizian T., Vosswinkel J.A., Davis J.E, & Jawa R.S. (2018). Vitamin D Binding Protein (DBP) Deficiency in Mice Decreases Systemic and Select Tissue Levels of Inflammatory Cytokines in a Murine Model of Acute Muscle Injury. The journal of trauma and acute care surgery, 84(6), 847-854.
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3

Multiplex Cytokine Quantification

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IL8, TNFα, and CCL3 concentrations were determined using Bio-Plex/Luminex assays (Bio-Rad). IL-1β and M-CSF were determined using ELISA assays (Thermo-Fisher, Waltham, MA).
Managò A., Audrito V., Mazzola F., Sorci L., Gaudino F., Gizzi K., Vitale N., Incarnato D., Minazzato G., Ianniello A., Varriale A., D’Auria S., Mengozzi G., Politano G., Oliviero S., Raffaelli N, & Deaglio S. (2019). Extracellular nicotinate phosphoribosyltransferase binds Toll like receptor 4 and mediates inflammation. Nature Communications, 10, 4116.
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4

Cytokine Profiling in CoCrMo Exposure

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IL-1β, IL-6, IL-10, IL-13, GM-CSF and TNFα were measured in plasma taken from CoCrMo treated whole blood using a Bio-Plex Luminex assay (Bio-Rad). All plasma samples were diluted in accordance with the manufacturer's recommendations.
Pearson M.J., Williams R.L., Floyd H., Bodansky D., Grover L.M., Davis E.T, & Lord J.M. (2015). The effects of cobalt-chromium-molybdenum wear debris in vitro on serum cytokine profiles and T cell repertoire. Biomaterials, 67.
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