The active pharmaceutical ingredient of epalrestat (purity > 99.5%) was obtained from Ningxia Kangya Pharmaceutical Industry (China). Acetonitrile and methanol of HPLC grade were purchased from Fisher (Fair lawn, NJ, USA). N,N-dimethyl formamide was obtained from Shandong Yuwang Industrial Co., Ltd (Shandong, China). Ammonium acetate (HPLC grade) was obtained from Dikma (Richmond Hill, NY, USA). Sodium dihydrogen phosphate, potassium phosphate monobasic and sodium phosphate dibasic dodecahydrate were purchased from Tianjin Kemiou Chemical Reagent Co., Ltd (Tianjin, China). Hydrochloric acid was obtained from Tianjin Kaixin Chemical Reagent Manufacturing Co., Ltd (Tianjin, China). H2O2 (30%), sodium hydroxide and sodium chloride were purchased from Tianjin Hengxing Chemical Reagent Manufacturing Co., Ltd (Tianjin, China). Ultra-pure water from Wahaha (Hangzhou, Zhejiang, China) was used. All other reagents and chemicals used were of analytical grade and are commercially available.
Hplc grade ammonium acetate
Manufactured by Dikma Technologies
Sourced in United States, China
HPLC-grade ammonium acetate is a high-purity chemical compound used as a buffer in high-performance liquid chromatography (HPLC) applications. It is a salt formed by the combination of acetic acid and ammonia. The compound is designed to meet the stringent purity requirements of HPLC analysis.
Automatically generated - may contain errors
Lab products found in correlation
Acetonitrile, by Thermo Fisher Scientific (2 mentions)
Methanol, by Thermo Fisher Scientific (2 mentions)
Formic acid, by Dikma Technologies (2 mentions)
Sodium dihydrogen phosphate, by Tianjin Kemiou Chemical Reagent (1 mentions)
Glipizide, by Pfizer (1 mentions)
Formic acid, by Thermo Fisher Scientific (1 mentions)
Isopropanol, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Hplc grade acetonitrile, by Merck Group (1 mentions)
Agilent 1200 hplc system, by Agilent Technologies (1 mentions)
Bifendate, by Chengdu Must Bio-Technology (1 mentions)
Milli q water purification system, by Merck Group (1 mentions)
5 protocols using hplc grade ammonium acetate
1
HPLC Quantification of Epalrestat Purity
2
Glipizide Tablet Formulation and Characterization
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glipizide tablets chosen as the reference (containing 5 mg glipizide in total weight of 200 mg, RLD) were purchased from Pfizer Co., Ltd. (Dalian, China). Two batches of glipizide were obtained from Weihai Disu Pharmaceuticals Co., Ltd. (Shandong, China). Reference standards of glipizide (99.5% purity) and gliclazide used as internal standard were obtained from the National Institute for Control of Pharmaceutical and Biological Products (Beijing, China). Lactose monohydrate was purchased from Jiangsu DaoNing Pharmaceutical Co., Ltd. (China). Microcrystalline cellulose, starch and colloidal silicon dioxide were gifted by Anhui Sunhere Pharmaceutical Excipients Co., Ltd.(China). Stearic acid was provided by Huzhou Zhan Wang Pharmaceutical Co., Ltd. (China). Methanol, acetonitrile and formic acid, all with HPLC grade were procured from Fisher (USA). Ammonium acetate (HPLC grade) was purchased from Dikma (Richmond Hill, NY, USA). Deionized-distilled water was used throughout the study.
3
HPLC Reagent Preparation Protocol
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HPLC-grade acetonitrile, isopropanol, and methanol were purchased from Merck (Darmstadt, Germany). HPLC-grade ammonium acetate and formic acid were purchased from DiKMA Technologies (Beijing, China). Ultrapure water was prepared using Elga’s water system (Wycombe, UK).
4
Quantitative Analysis of Bioactive Compounds
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An Agilent 1200 HPLC system (Agilent, Palo Alto, CA, USA), equipped with a quaternary solvent delivery pump, an autosampler and a column oven, was used. A 3200 QTRAP™ mass spectrometer system with anESI source (AB SCIEX, CA, USA) was used. Analyst™ software (versions 1.5.2) was used for the data acquisition and processing.
Harpagide ( purity ≥98%), notoginsenoside R1 ( purity ≥98%), ginsenoside Rg1 ( purity ≥98%), ginsenoside Rb1 ( purity ≥98%), salvianolic acid B ( purity ≥95%), harpagoside ( purity ≥98%), astragaloside IV ( purity ≥98%) and tanshinone IIA ( purity ≥98%) were purchased from the National Institutes for Food and Drug Control (Beijing, China). Fufang Xueshuangtong capsules (batch number, 121108, 130314 and 130830) were purchased from Guangdong Zhongsheng Pharmacy Co., Ltd (Guangdong, China).
HPLC-grade acetonitrile and methanol were purchased from J.T. Baker (Center Valley, PA, USA). HPLC-grade ammonium acetate and formic acid were obtained from Dikma Technologies Inc. (Lake Forest, CA, USA). Deionized water was purified by a Milli-Q plot water purification system (MA, USA).
Harpagide ( purity ≥98%), notoginsenoside R1 ( purity ≥98%), ginsenoside Rg1 ( purity ≥98%), ginsenoside Rb1 ( purity ≥98%), salvianolic acid B ( purity ≥95%), harpagoside ( purity ≥98%), astragaloside IV ( purity ≥98%) and tanshinone IIA ( purity ≥98%) were purchased from the National Institutes for Food and Drug Control (Beijing, China). Fufang Xueshuangtong capsules (batch number, 121108, 130314 and 130830) were purchased from Guangdong Zhongsheng Pharmacy Co., Ltd (Guangdong, China).
HPLC-grade acetonitrile and methanol were purchased from J.T. Baker (Center Valley, PA, USA). HPLC-grade ammonium acetate and formic acid were obtained from Dikma Technologies Inc. (Lake Forest, CA, USA). Deionized water was purified by a Milli-Q plot water purification system (MA, USA).
5
Isolation and Characterization of Bioactive Standards from Rubia cordifolia
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Reference standards of purpurin, munjistin, and mollugin were isolated from R. cordifolia and identified by our laboratory. On the basis of UV, MS, NMR and HPLC analysis, the chemical structures and the purities (purity ≥ 98%) of isolated reference standards were confirmed. Bifendate (Lots: 100192-200503) was purchased from Chengdu Must Bio-Technology Co., Ltd. (Chengdu, Sichuan, China) and used as an internal standard (I.S.).
The following solvents and chemicals were used in our experiments: J & K Medical (Beijing, China) provided the HPLC-grade methanol (MeOH) and acetonitrile (CH3CN). HPLC-grade ammonium acetate was purchased from DIKMA Technologies (Beijing, China). All other reagents were of analytical-grade. Ultrapure water was prepared using a Milli-Q water purification system (Millipore, Molsheim, France).
The dried roots and rhizomes of R. cordifolia were obtained from Bozhou (Anhui, China), the largest Chinese herbal medicine distribution center in the world, and were identified by Professor Zhenyue Wang in Heilongjiang University of Chinese Medicine. A voucher specimen (Art. No. 20160001) was deposited in the College of Pharmacy, Harbin Medical University, China.
The following solvents and chemicals were used in our experiments: J & K Medical (Beijing, China) provided the HPLC-grade methanol (MeOH) and acetonitrile (CH3CN). HPLC-grade ammonium acetate was purchased from DIKMA Technologies (Beijing, China). All other reagents were of analytical-grade. Ultrapure water was prepared using a Milli-Q water purification system (Millipore, Molsheim, France).
The dried roots and rhizomes of R. cordifolia were obtained from Bozhou (Anhui, China), the largest Chinese herbal medicine distribution center in the world, and were identified by Professor Zhenyue Wang in Heilongjiang University of Chinese Medicine. A voucher specimen (Art. No. 20160001) was deposited in the College of Pharmacy, Harbin Medical University, China.
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